Overview

  • Product nameAnti-Integrin beta 5 antibody
    See all Integrin beta 5 primary antibodies
  • Description
    Rabbit polyclonal to Integrin beta 5
  • Specificityab53111 detects endogenous levels of total Integrin beta 5 protein.
  • Tested applicationsSuitable for: WB, ELISA, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide corresponding to Human Integrin beta 5. Immunogen range: aa 721-770
    Database link: P18084

  • Positive control
    • Extracts from COS7 cells.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesab53111 was affinity purified from rabbit antiserum by affinity chromatography using epitope-specific immunogen.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab53111 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/300 - 1/1000. Detects a band of approximately 88 kDa (predicted molecular weight: 88 kDa).
ELISA 1/20000.
ICC/IF Use at an assay dependent concentration. PubMed: 22428014

Target

Anti-Integrin beta 5 antibody images

  • All lanes : Anti-Integrin beta 5 antibody (ab53111) at 1/300 dilution

    Lane 1 : extract from COS7 cells
    Lane 2 : extract from COS7 cells with immunising peptide


    Predicted band size : 88 kDa
    Observed band size : 88 kDa

References for Anti-Integrin beta 5 antibody (ab53111)

This product has been referenced in:
  • Becker L  et al. Unique proteomic signatures distinguish macrophages and dendritic cells. PLoS One 7:e33297 (2012). ICC/IF ; Mouse . Read more (PubMed: 22428014) »
  • Miranda A  et al. Prion protein expression regulates embryonic stem cell pluripotency and differentiation. PLoS One 6:e18422 (2011). Read more (PubMed: 21483752) »

See all 2 Publications for this product

Product Wall

Thank you for your enquiry.

The integrin beta 5protein is known to be glycosylated. Some other integrin beta 5 antibodies do show a ˜100 kDa and this is probably the glycosylated form.

The following Uniprot protein database entry ...

Read More
Abcam has not validated the combination of species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (Mouse Embryonic Fibroblast (MEF))
Specification Mouse Embryonic Fibroblast (MEF)
Fixative Paraformaldehyde
Permeabilization Yes - triton
Username

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Verified customer

Submitted Nov 16 2011

Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (HeLa cell line ( cervical cancer))
Specification HeLa cell line ( cervical cancer)
Fixative Paraformaldehyde
Permeabilization Yes - triton
Username

Abcam user community

Verified customer

Submitted Nov 16 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"