Anti-Integrin beta 7 antibody (ab169300)

Overview

  • Product name
    Anti-Integrin beta 7 antibody
    See all Integrin beta 7 primary antibodies
  • Description
    Mouse polyclonal to Integrin beta 7
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rabbit, Horse, Cow, Pig, Chimpanzee, Cynomolgus monkey, Rhesus monkey, Gorilla, Common marmoset
  • Immunogen

    Full length protein, corresponding to amino acids 1-798 of Human Integrin beta 7 (NP_000880.1).

  • Positive control
    • Integrin beta 7 transfected 293T cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab169300 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 87 kDa.

Target

  • Function
    Integrin alpha-4/beta-7 (Peyer patches-specific homing receptor LPAM-1) is an adhesion molecule that mediates lymphocyte migration and homing to gut-associated lymphoid tissue (GALT). Integrin alpha-4/beta-7 interacts with the cell surface adhesion molecules MADCAM1 which is normally expressed by the vascular endothelium of the gastrointestinal tract. Interacts also with VCAM1 and fibronectin, an extracellular matrix component. It recognizes one or more domains within the alternatively spliced CS-1 region of fibronectin. Interactions involves the tripeptide L-D-T in MADCAM1, and L-D-V in fibronectin. Binds to HIV-1 gp120, thereby allowing the virus to enter GALT, which is thought to be the major trigger of AIDS disease. Interaction would involve a tripeptide L-D-I in HIV-1 gp120. Integrin alpha-E/beta-7 (HML-1) is a receptor for E-cadherin.
  • Tissue specificity
    Expressed in a variety of leukocyte lines.
  • Sequence similarities
    Belongs to the integrin beta chain family.
    Contains 1 VWFA domain.
  • Domain
    Domain I contains three cation-binding sites: the ligand-integrin-binding site (LIMBS), the metal ion-dependent adhesion site (MIDAS), and the adjacent to MIDAS site (ADMIDAS). In the absence of a ligand or in calcium-dependent binding, only ADMIDAS is occupied. In magnesium-dependent binding all three sites bind metal ions. LIMBS positively modify ligand binding whereas ADMIDAS negatively modify ligand binding.
  • Cellular localization
    Membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Gut homing receptor beta subunit antibody
    • Integrin beta 7 subunit antibody
    • Integrin beta-7 antibody
    • Integrin beta-P antibody
    • Integrin, beta 7 antibody
    • ITB7 antibody
    • ITB7_HUMAN antibody
    • Itgb7 antibody
    • Ly69 antibody
    • M290 IEL antigen antibody
    see all

Images

  • All lanes : Anti-Integrin beta 7 antibody (ab169300) at 1 µg/ml

    Lane 1 : Integrin beta 7 transfected 293T cell lysate
    Lane 2 : Non transfected 293T cell lysate

    Lysates/proteins at 15 µl per lane.

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP at 1/2500 dilution
    Developed using the ECL technique

    Predicted band size : 87 kDa

References

ab169300 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Pig Tissue sections (intestine)
Permeabilization
No
Specification
intestine
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 2% · Temperature: 25°C
Fixative
Acetone
Username

Dr. Abdul Rauf

Verified customer

Submitted Sep 05 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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