Anti-Integrin beta 7 antibody [FIB504] (Biotin) (ab95816)


  • Product name
    Anti-Integrin beta 7 antibody [FIB504] (Biotin)
    See all Integrin beta 7 primary antibodies
  • Description
    Rat monoclonal [FIB504] to Integrin beta 7 (Biotin)
  • Conjugation
  • Tested applications
    Suitable for: ICC/IF, Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    TK1 murine t-cell lymphoma

  • Positive control
    • Normal Human peripheral blood cells



Our Abpromise guarantee covers the use of ab95816 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/300.
Flow Cyt Use 0.125µg for 105 cells. Range from 105 to 108 cells/test in a final volume of 100 µl.

ab18445-Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.


  • Function
    Integrin alpha-4/beta-7 (Peyer patches-specific homing receptor LPAM-1) is an adhesion molecule that mediates lymphocyte migration and homing to gut-associated lymphoid tissue (GALT). Integrin alpha-4/beta-7 interacts with the cell surface adhesion molecules MADCAM1 which is normally expressed by the vascular endothelium of the gastrointestinal tract. Interacts also with VCAM1 and fibronectin, an extracellular matrix component. It recognizes one or more domains within the alternatively spliced CS-1 region of fibronectin. Interactions involves the tripeptide L-D-T in MADCAM1, and L-D-V in fibronectin. Binds to HIV-1 gp120, thereby allowing the virus to enter GALT, which is thought to be the major trigger of AIDS disease. Interaction would involve a tripeptide L-D-I in HIV-1 gp120. Integrin alpha-E/beta-7 (HML-1) is a receptor for E-cadherin.
  • Tissue specificity
    Expressed in a variety of leukocyte lines.
  • Sequence similarities
    Belongs to the integrin beta chain family.
    Contains 1 VWFA domain.
  • Domain
    Domain I contains three cation-binding sites: the ligand-integrin-binding site (LIMBS), the metal ion-dependent adhesion site (MIDAS), and the adjacent to MIDAS site (ADMIDAS). In the absence of a ligand or in calcium-dependent binding, only ADMIDAS is occupied. In magnesium-dependent binding all three sites bind metal ions. LIMBS positively modify ligand binding whereas ADMIDAS negatively modify ligand binding.
  • Cellular localization
  • Information by UniProt
  • Database links
  • Alternative names
    • Gut homing receptor beta subunit antibody
    • Integrin beta 7 subunit antibody
    • Integrin beta-7 antibody
    • Integrin beta-P antibody
    • Integrin, beta 7 antibody
    • ITB7 antibody
    • ITB7_HUMAN antibody
    • Itgb7 antibody
    • Ly69 antibody
    • M290 IEL antigen antibody
    see all

Anti-Integrin beta 7 antibody [FIB504] (Biotin) images

  • ICC/IF image of Integrin beta 7 staining on RAW264.7 cell line using ab95816 (1:300). The cells were fixed using formaldehyde and permeabilized using 0.2% Triton X-100. The cells were blocked using 10% goat serum for 1 hour at 20°C. The cells were incubated with ab95816 (1:300) for 1 hour at 20°C. The staining was detected using Streptavedin conjugated to Alexo Fluor 647 (1:350)

    See Abreview

  • Staining of normal Human peripheral blood cells with 0.06 µg of ab95816 followed by Streptavidin PE. Cells in the lymphocyte gate were used for analysis.
  • Staining of normal Human peripheral blood cells with Anti-Human CD3 APC and 0.125 µg of Rat IgG2a ? Isotype Control Biotin. Cells in the lymphocyte gate were used for analysis.

References for Anti-Integrin beta 7 antibody [FIB504] (Biotin) (ab95816)

This product has been referenced in:
  • Meerschaert J  et al. Engagement of alpha4beta7 integrins by monoclonal antibodies or ligands enhances survival of human eosinophils in vitro. J Immunol 163:6217-27 (1999). Read more (PubMed: 10570314) »
  • Tidswell M  et al. Structure-function analysis of the integrin beta 7 subunit: identification of domains involved in adhesion to MAdCAM-1. J Immunol 159:1497-505 (1997). Read more (PubMed: 9233649) »

See all 2 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Immunocytochemistry/ Immunofluorescence
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C
Mouse Cell (raw 264.7 (macrophage))
raw 264.7 (macrophage)
Yes - 0.2% Triton X-100

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Submitted Jul 01 2013


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