Anti-Integrin beta 7 antibody [FIB504] (Phycoerythrin) (ab95817)

Overview

  • Product name
    Anti-Integrin beta 7 antibody [FIB504] (Phycoerythrin)
    See all Integrin beta 7 primary antibodies
  • Description
    Rat monoclonal [FIB504] to Integrin beta 7 (Phycoerythrin)
  • Conjugation
    Phycoerythrin. Ex: 488nm, Em: 575nm
  • Tested applications
    Suitable for: Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    TK1 murine t-cell lymphoma

  • Positive control
    • Normal Human peripheral blood cells
  • General notes
    1 test = 5 microlitres

Properties

Applications

Our Abpromise guarantee covers the use of ab95817 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 5µl for 106 cells.

Target

  • Function
    Integrin alpha-4/beta-7 (Peyer patches-specific homing receptor LPAM-1) is an adhesion molecule that mediates lymphocyte migration and homing to gut-associated lymphoid tissue (GALT). Integrin alpha-4/beta-7 interacts with the cell surface adhesion molecules MADCAM1 which is normally expressed by the vascular endothelium of the gastrointestinal tract. Interacts also with VCAM1 and fibronectin, an extracellular matrix component. It recognizes one or more domains within the alternatively spliced CS-1 region of fibronectin. Interactions involves the tripeptide L-D-T in MADCAM1, and L-D-V in fibronectin. Binds to HIV-1 gp120, thereby allowing the virus to enter GALT, which is thought to be the major trigger of AIDS disease. Interaction would involve a tripeptide L-D-I in HIV-1 gp120. Integrin alpha-E/beta-7 (HML-1) is a receptor for E-cadherin.
  • Tissue specificity
    Expressed in a variety of leukocyte lines.
  • Sequence similarities
    Belongs to the integrin beta chain family.
    Contains 1 VWFA domain.
  • Domain
    Domain I contains three cation-binding sites: the ligand-integrin-binding site (LIMBS), the metal ion-dependent adhesion site (MIDAS), and the adjacent to MIDAS site (ADMIDAS). In the absence of a ligand or in calcium-dependent binding, only ADMIDAS is occupied. In magnesium-dependent binding all three sites bind metal ions. LIMBS positively modify ligand binding whereas ADMIDAS negatively modify ligand binding.
  • Cellular localization
    Membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Gut homing receptor beta subunit antibody
    • Integrin beta 7 subunit antibody
    • Integrin beta-7 antibody
    • Integrin beta-P antibody
    • Integrin, beta 7 antibody
    • ITB7 antibody
    • ITB7_HUMAN antibody
    • Itgb7 antibody
    • Ly69 antibody
    • M290 IEL antigen antibody
    see all

Images

  • Staining of normal Human peripheral blood cells with Rat IgG2a ? Isotype Control PE (open histogram) or ab95817 (filled histogram). Cells in the lymphocyte gate were used for analysis.

References

This product has been referenced in:
  • Meerschaert J  et al. Engagement of alpha4beta7 integrins by monoclonal antibodies or ligands enhances survival of human eosinophils in vitro. J Immunol 163:6217-27 (1999). Read more (PubMed: 10570314) »

See 1 Publication for this product

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab95817.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up