Anti-Integrin beta 8 antibody (ab80673)


  • Product nameAnti-Integrin beta 8 antibody
    See all Integrin beta 8 primary antibodies
  • Description
    Rabbit polyclonal to Integrin beta 8
  • Tested applicationsSuitable for: ELISA, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Rat, Human
    Predicted to work with: Mouse, Rabbit, Horse, Guinea pig, Cow, Cat, Dog, Pig
  • Immunogen

    Synthetic peptide: CECTDPRSIG RFCEHCPTCY TACKENWNCM QCLHPHNLSQ AILDQCKTSC , corresponding to C terminal amino acids 611-660 of Human Integrin beta 8 (accession number: NP_002205)

  • Positive control
    • Human placenta membrane lysate This antibody gave a positive result in IHC in the following FFPE tissue: Human normal placenta.


  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • Storage bufferConstituents: 2% Sucrose, 98% PBS
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas


Our Abpromise guarantee covers the use of ab80673 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.

Only Peptide ELISA tested

IHC-P Use a concentration of 1 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 81 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.


Anti-Integrin beta 8 antibody images

  • IHC image of Integrin beta 8 staining in human placenta formalin fixed paraffin embedded tissue section*, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab80673, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

  • Anti-Integrin beta 8 antibody (ab80673) at 1 µg/ml + Placenta lysate at 10 µg

    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Observed band size : 81 kDa (why is the actual band size different from the predicted?)

References for Anti-Integrin beta 8 antibody (ab80673)

ab80673 has not yet been referenced specifically in any publications.

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Glioma)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Tris/EDTA buffer, pH 9
Permeabilization No
Specification Glioma
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 25°C
Fixative Paraformaldehyde

Abcam user community

Verified customer

Submitted Jun 11 2015

Application Western blot
Loading amount 400000 cells
Gel Running Conditions Reduced Denaturing (10%)
Sample Rat Cell lysate - whole cell (primary astrocytes)
Specification primary astrocytes
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

Abcam user community

Verified customer

Submitted Dec 22 2014

Application Immunocytochemistry/ Immunofluorescence
Blocking step Serum as blocking agent for 20 minute(s) · Concentration: 10% · Temperature: 22°C
Sample Rat Cell (primary brain astrocytes)
Specification primary brain astrocytes
Permeabilization Yes - 0,1% Triton
Fixative Paraformaldehyde

Abcam user community

Verified customer

Submitted Dec 19 2014

Application Western blot
Loading amount 30 µg
Gel Running Conditions Reduced Denaturing (10% gel)
Sample Human Cell lysate - whole cell (Glioma)
Specification Glioma
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 20°C

Abcam user community

Verified customer

Submitted Dec 18 2013

Unfortunately this antibody has not yet been tested in Flow Cytometry, so we don't have any data stating whether or not this antibody would be suitable for this application.

I am very pleased to hear you would like to accept our offer and test ab80673 in WB in mouse. This code will give you 1 freeprimary antibody before the expiration date. To redeem this offer, please submit an Abreview for WB in mouse and include this co...

Read More