Overview

  • Product name
    Anti-Integrin beta 8 antibody
    See all Integrin beta 8 primary antibodies
  • Description
    Rabbit polyclonal to Integrin beta 8
  • Tested applications
    Suitable for: ELISA, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Rat, Human
    Predicted to work with: Mouse, Rabbit, Horse, Guinea pig, Cow, Cat, Dog, Pig
  • Immunogen

    Synthetic peptide: CECTDPRSIG RFCEHCPTCY TACKENWNCM QCLHPHNLSQ AILDQCKTSC , corresponding to C terminal amino acids 611-660 of Human Integrin beta 8 (accession number: NP_002205)

  • Positive control
    • Human placenta membrane lysate This antibody gave a positive result in IHC in the following FFPE tissue: Human normal placenta.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Constituents: 2% Sucrose, 98% PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab80673 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.

Only Peptide ELISA tested

IHC-P Use a concentration of 1 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 81 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

Anti-Integrin beta 8 antibody images

  • IHC image of Integrin beta 8 staining in human placenta formalin fixed paraffin embedded tissue section*, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab80673, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

  • Anti-Integrin beta 8 antibody (ab80673) at 1 µg/ml + Placenta lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Observed band size : 81 kDa (why is the actual band size different from the predicted?)

References for Anti-Integrin beta 8 antibody (ab80673)

This product has been referenced in:
  • Bian Q  et al. Mechanosignaling activation of TGFß maintains intervertebral disc homeostasis. Bone Res 5:17008 (2017). IHC-P ; Mouse . Read more (PubMed: 28392965) »
  • Mertens-Walker I  et al. The tumour-promoting receptor tyrosine kinase, EphB4, regulates expression of integrin-ß8 in prostate cancer cells. BMC Cancer 15:164 (2015). WB . Read more (PubMed: 25886373) »

See all 2 Publications for this product

Product Wall

Unfortunately this antibody has not yet been tested in Flow Cytometry, so we don't have any data stating whether or not this antibody would be suitable for this application.

I am very pleased to hear you would like to accept our offer and test ab80673 in WB in mouse. This code will give you 1 freeprimary antibody before the expiration date. To redeem this offer, please submit an Abreview for WB in mouse and include this co...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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