Overview

  • Product name
    Anti-Integrin linked ILK antibody
    See all Integrin linked ILK primary antibodies
  • Description
    Goat polyclonal to Integrin linked ILK
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Guinea pig
  • Immunogen

    Synthetic peptide: DMIVPILEKMQDK, corresponding to C terminal amino acids 440-452 of Human Integrin linked ILK.

  • Positive control
    • A431 lysate.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, 0.5mg/ml Tris saline. pH 7.3
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab15838 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 52 kDa).Can be blocked with Human Integrin linked ILK peptide (ab23275).

Target

  • Function
    Receptor-proximal protein kinase regulating integrin-mediated signal transduction. May act as a mediator of inside-out integrin signaling. Focal adhesion protein part of the complex ILK-PINCH. This complex is considered to be one of the convergence points of integrin- and growth factor-signaling pathway. Could be implicated in mediating cell architecture, adhesion to integrin substrates and anchorage-dependent growth in epithelial cells. Phosphorylates beta-1 and beta-3 integrin subunit on serine and threonine residues, but also AKT1 and GSK3B.
  • Tissue specificity
    Highly expressed in heart followed by skeletal muscle, pancreas and kidney. Weakly expressed in placenta, lung and liver.
  • Sequence similarities
    Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family.
    Contains 5 ANK repeats.
    Contains 1 protein kinase domain.
  • Domain
    A PH-like domain is involved in phosphatidylinositol phosphate binding.
  • Post-translational
    modifications
    Autophosphorylated on serine residues.
  • Cellular localization
    Cell junction > focal adhesion. Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • 59 kDa serine/threonine protein kinase antibody
    • 59 kDa serine/threonine-protein kinase antibody
    • DKFZp686F1765 antibody
    • Epididymis secretory protein Li 28 antibody
    • HEL S 28 antibody
    • ILK 1 antibody
    • ILK 2 antibody
    • ILK antibody
    • ILK-1 antibody
    • ILK-2 antibody
    • ILK_HUMAN antibody
    • ILK1 antibody
    • ILK2 antibody
    • Integrin linked kinase 2 antibody
    • Integrin linked Kinase antibody
    • Integrin linked protein kinase antibody
    • Integrin-linked protein kinase antibody
    • p59 antibody
    • p59ILK antibody
    see all

Images



  • Predicted band size : 52 kDa


    ab15838 staining human Integrin linked ILK at 0.5µg/ml in A431 lysate (35µg) by Western blot (chemiluminsecence).

References

This product has been referenced in:
  • Dong Z  et al. Activated Integrin-Linked Kinase Negatively Regulates Muscle Cell Enhancement Factor 2C in C2C12 Cells. Biomed Res Int 2015:748470 (2015). Read more (PubMed: 26788505) »
  • Pillozzi S  et al. Chemotherapy resistance in acute lymphoblastic leukemia requires hERG1 channels and is overcome by hERG1 blockers. Blood 117:902-14 (2011). WB . Read more (PubMed: 21048156) »

See all 2 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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