FunctionReceptor-proximal protein kinase regulating integrin-mediated signal transduction. May act as a mediator of inside-out integrin signaling. Focal adhesion protein part of the complex ILK-PINCH. This complex is considered to be one of the convergence points of integrin- and growth factor-signaling pathway. Could be implicated in mediating cell architecture, adhesion to integrin substrates and anchorage-dependent growth in epithelial cells. Phosphorylates beta-1 and beta-3 integrin subunit on serine and threonine residues, but also AKT1 and GSK3B.
Tissue specificityHighly expressed in heart followed by skeletal muscle, pancreas and kidney. Weakly expressed in placenta, lung and liver.
Sequence similaritiesBelongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. Contains 5 ANK repeats. Contains 1 protein kinase domain.
DomainA PH-like domain is involved in phosphatidylinositol phosphate binding.
Post-translational modificationsAutophosphorylated on serine residues.
Immunocytochemistry/ Immunofluorescence - Anti-Integrin linked ILK antibody (ab74336)
Immunocytochemistry/Immunofluorescence analysis of acetone-fixed asynchronous HeLa cells labelling Integrin linked ILK with ab74336 at 1/100 (2µg/ml). A red-fluorescent goat anti-rabbit IgG (1/100) was used as the secondary antibody.
Western blot - Integrin linked ILK antibody (ab74336)
All lanes : Anti-Integrin linked ILK antibody (ab74336) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg Lane 2 : HeLa whole cell lysate at 15 µg Lane 3 : HeLa whole cell lysate at 5 µg Lane 4 : 293T whole cell lysate at 50 µg Lane 5 : NIH3T3 whole cell lysate at 50 µg
Developed using the ECL technique
Predicted band size : 51 kDa Observed band size : 51 kDa
Exposure time : 10 seconds
Immunoprecipitation - Integrin linked ILK antibody (ab74336)
Detection of Integrin linked ILK by Western Blot of Immunprecipitate.
ab74336 at 1µg/ml staining Integrin linked ILK in HeLa whole cell lysate immunoprecipitated using ab74336 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Detection: Chemiluminescence with exposure time of 10 seconds.
Immunocytochemistry/ Immunofluorescence - Integrin linked ILK antibody (ab74336)
ICC/IF image of ab74336 stained MCF7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab74336, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
References for Anti-Integrin linked ILK antibody (ab74336)
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