Anti-Integrin linked ILK antibody [EPR1592] (ab76468)

Overview

  • Product nameAnti-Integrin linked ILK antibody [EPR1592]
    See all Integrin linked ILK primary antibodies
  • Description
    Rabbit monoclonal [EPR1592] to Integrin linked ILK
  • Tested applicationsSuitable for: WB, Flow Cyt, ICC/IFmore details
    Unsuitable for: IHC-P or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human Integrin linked ILK.

  • Positive control
    • HeLa, 293T, A431 and Jurkat cell lysates; HeLa cells.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

     

    Alternative versions available:

    Anti-Integrin linked ILK antibody (Alexa Fluor® 647) [EPR1592] (ab196851)

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage bufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
  • PurityTissue culture supernatant
  • ClonalityMonoclonal
  • Clone numberEPR1592
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab76468 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/5000 - 1/50000. Detects a band of approximately 51 kDa (predicted molecular weight: 51 kDa).
Flow Cyt 1/100. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
ICC/IF 1/500.

For unpurified use at 1/100 - 1/250

  • Application notesIs unsuitable for IHC-P or IP.
  • Target

    • FunctionReceptor-proximal protein kinase regulating integrin-mediated signal transduction. May act as a mediator of inside-out integrin signaling. Focal adhesion protein part of the complex ILK-PINCH. This complex is considered to be one of the convergence points of integrin- and growth factor-signaling pathway. Could be implicated in mediating cell architecture, adhesion to integrin substrates and anchorage-dependent growth in epithelial cells. Phosphorylates beta-1 and beta-3 integrin subunit on serine and threonine residues, but also AKT1 and GSK3B.
    • Tissue specificityHighly expressed in heart followed by skeletal muscle, pancreas and kidney. Weakly expressed in placenta, lung and liver.
    • Sequence similaritiesBelongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family.
      Contains 5 ANK repeats.
      Contains 1 protein kinase domain.
    • DomainA PH-like domain is involved in phosphatidylinositol phosphate binding.
    • Post-translational
      modifications
      Autophosphorylated on serine residues.
    • Cellular localizationCell junction > focal adhesion. Cell membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • 59 kDa serine/threonine protein kinase antibody
      • 59 kDa serine/threonine-protein kinase antibody
      • DKFZp686F1765 antibody
      • Epididymis secretory protein Li 28 antibody
      • HEL S 28 antibody
      • ILK 1 antibody
      • ILK 2 antibody
      • ILK antibody
      • ILK-1 antibody
      • ILK-2 antibody
      • ILK_HUMAN antibody
      • ILK1 antibody
      • ILK2 antibody
      • Integrin linked kinase 2 antibody
      • Integrin linked Kinase antibody
      • Integrin linked protein kinase antibody
      • Integrin-linked protein kinase antibody
      • p59 antibody
      • p59ILK antibody
      see all

    Anti-Integrin linked ILK antibody [EPR1592] images



    • Predicted band size : 51 kDa

      Lane 1: Wild-type HAP1 cell lysate (20 µg)
      Lane 2: Integrin linked ILK knockout HAP1 cell lysate (20 µg)
      Lane 3: Hek293 cell lysate (20 µg)
      Lane 4: K562 cell lysate (20 µg)
      Lanes 1 - 4: Merged signal (red and green). Green - ab76468 observed at 51 kDa. Red - loading control, ab8245, observed at 37 kDa.

      ab76468 was shown to specifically react with Integrin linked ILK when Integrin linked ILK knockout samples were used. Wild-type and Integrin linked ILK knockout samples were subjected to SDS-PAGE. ab76468 and ab8245 (loading control to GAPDH) were diluted at 1/5000 and 1/10 000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) ab216776 secondary antibodies at 1/10000 dilution for 1 h at room temperature before imaging.

    • Immunocytochemistry/Immunofluorescence analysis of A431 (human epidermoid carcinoma) cells labelling Integrin linked ILK (green) with purified ab76468 at 1/500. Cells were fixed with 100% methanol. ab150077, Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. Nuclei were stained blue with DAPI.  

      Secondary Only Control: PBS was used instead of the primary antibody as the negative control.

    • All lanes : Anti-Integrin linked ILK antibody [EPR1592] (ab76468) at 1/50000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : 293T cell lysate
      Lane 3 : Jurkat cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size : 51 kDa
      Observed band size : 51 kDa
    • ab76468 at 1/100 dilution staining Integrin linked ILK in HeLa cells by Immunofluorescence.
    • ab76468 at 1/100 dilution staining Integrin linked ILK in HeLa cells by Immunofluorescence.
    • Overlay histogram showing HEK293 cells stained with ab76468 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab76468, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was a goat anti-rabbit DyLight® 488 (IgG; H+L) (ab96899) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1 µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HEK293 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

    References for Anti-Integrin linked ILK antibody [EPR1592] (ab76468)

    ab76468 has not yet been referenced specifically in any publications.

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Human Cell lysate - whole cell (platelet)
    Loading amount 25 µg
    Specification platelet
    Gel Running Conditions Reduced Denaturing
    Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 20°C
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    Submitted Jan 04 2012

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"