Anti-Interferon alpha 1 antibody [F18] (ab7373)
Key features and details
- Rat monoclonal [F18] to Interferon alpha 1
- Suitable for: Neutralising, Flow Cyt, Inhibition Assay
- Reacts with: Mouse
- Isotype: IgG1
Overview
-
Product name
Anti-Interferon alpha 1 antibody [F18]
See all Interferon alpha 1 primary antibodies -
Description
Rat monoclonal [F18] to Interferon alpha 1 -
Host species
Rat -
Specificity
This monoclonal antibody binds and neutralizes both natural and recombinant mouse alpha Interferon 1. This antibody does not cross-react with Human Interferon, Mouse Interferon beta or Mouse Interferon gamma. -
Tested applications
Suitable for: Neutralising, Flow Cyt, Inhibition Assaymore details -
Species reactivity
Reacts with: Mouse
Does not react with: Human -
Positive control
- Plasmacytoid dendritic cells.
-
General notes
With the availability of monoclonal antibodies directed against alpha Interferon, it is possible to interpret results obtained from crude materials containing both alpha and beta Interferon. The difficulties in studying in vitro and in vivo effects of "type 1" Interferons arise from the fact that both alpha and beta Interferons are produced in response to the same stimuli and also seem to act via the same receptor. These Interferon activities can only be distinguished from one another by use of specific neutralizing antibodies.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
Preservative: 0.1% Sodium azide
Constituents: 0.1% BSA, PBS -
Concentration information loading...
-
Purity
Protein G purified -
Purification notes
0.2 µm filtered antibody solution. -
Primary antibody notes
With the availability of monoclonal antibodies directed against alpha Interferon, it is possible to interpret results obtained from crude materials containing both alpha and beta Interferon. The difficulties in studying in vitro and in vivo effects of "type 1" Interferons arise from the fact that both alpha and beta Interferons are produced in response to the same stimuli and also seem to act via the same receptor. These Interferon activities can only be distinguished from one another by use of specific neutralizing antibodies. -
Clonality
Monoclonal -
Clone number
F18 -
Isotype
IgG1 -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab7373 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
Neutralising |
Use at an assay dependent concentration.
|
|
Flow Cyt |
Use at an assay dependent concentration. PubMed: 21172865
ab18407 - Rat monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
|
Inhibition Assay |
Use at an assay dependent concentration.
Useful for inhiiton in biological assays and for affinity pruification. |
|
AP |
Use at an assay dependent concentration.
|
Notes |
---|
Neutralising
Use at an assay dependent concentration. |
Flow Cyt
Use at an assay dependent concentration. PubMed: 21172865 ab18407 - Rat monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
Inhibition Assay
Use at an assay dependent concentration. Useful for inhiiton in biological assays and for affinity pruification. |
AP
Use at an assay dependent concentration. |
Target
-
Function
Produced by macrophages, IFN-alpha have antiviral activities. Interferon stimulates the production of two enzymes: a protein kinase and an oligoadenylate synthetase. -
Sequence similarities
Belongs to the alpha/beta interferon family. -
Cellular localization
Secreted. - Information by UniProt
-
Database links
- Entrez Gene: 15962 Mouse
- Entrez Gene: 230396 Mouse
- SwissProt: P01572 Mouse
-
Alternative names
- IFL antibody
- IFN alpha antibody
- IFN alpha 1/13 antibody
see all
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
-
SDS download
-
Datasheet download
References (7)
ab7373 has been referenced in 7 publications.
- Tian J et al. 5-Fluorouracil efficacy requires anti-tumor immunity triggered by cancer-cell-intrinsic STING. EMBO J 40:e106065 (2021). PubMed: 33615517
- Büssow K et al. ER intrabody-mediated inhibition of interferon a secretion by mouse macrophages and dendritic cells. PLoS One 14:e0215062 (2019). PubMed: 30990863
- Bi E et al. E-cadherin expression on multiple myeloma cells activates tumor-promoting properties in plasmacytoid DCs. J Clin Invest 128:4821-4831 (2018). PubMed: 30277474
- Biswas I et al. Extracellular RNA facilitates hypoxia-induced leukocyte adhesion and infiltration in the lung through TLR3-IFN-?-STAT1 signaling pathway. Eur J Immunol 45:3158-73 (2015). PubMed: 26350442
- Castellaneta A et al. Plasmacytoid dendritic cell-derived IFN-a promotes murine liver ischemia/reperfusion injury by induction of hepatocyte IRF-1. Hepatology 60:267-77 (2014). PubMed: 24493010
- Björck P et al. Plasmacytoid dendritic cell dichotomy: identification of IFN-a producing cells as a phenotypically and functionally distinct subset. J Immunol 186:1477-85 (2011). Flow Cyt ; Mouse . PubMed: 21172865
- Joshi VD et al. A role for Stat1 in the regulation of lipopolysaccharide-induced interleukin-1beta expression. J Interferon Cytokine Res 26:739-47 (2006). PubMed: 17032168