Anti-Interferon alpha 2b antibody [4E10] (ab9388)


  • Product nameAnti-Interferon alpha 2b antibody [4E10]
    See all Interferon alpha 2b primary antibodies
  • Description
    Mouse monoclonal [4E10] to Interferon alpha 2b
  • SpecificityRecognizes both human recombinant IFN-a2b and IFN-a2a. Does not react with human recombinant IFN-a5 and IFN-g. Does not inhibit antiviral activity of IFN-a2b in vitro.
  • Tested applicationsSuitable for: Indirect ELISA, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant full length protein (Human).

  • Positive control
  • General notesThe neutralising bioassay was performed using L41 cell line and mouse encephalomyocarditis virus (EMCV). Briefly, interferon was pre-incubated with the test antibodies for 2 h at RT and then added to the cell monolayer. 24 h later the cells were infected with EMCV and cultured for further 24 h. Antiviral activity of interferon was evaluated using crystal-violet staining. Polyclonal antibodies to respective interferons and irrelevant monoclonal antibody of the same isotype were used as controls.


  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 0.1% Sodium Azide
    Constituents: PBS, pH 7.4
  • Concentration information loading...
  • PurityIgG fraction
  • ClonalityMonoclonal
  • Clone number4E10
  • MyelomaNS0/1
  • IsotypeIgG1
  • Light chain typeunknown
  • Research areas


Our Abpromise guarantee covers the use of ab9388 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Indirect ELISA 1/500 - 1/2000.
WB 1/500.


  • FunctionProduced by macrophages, IFN-alpha have antiviral activities.
  • Sequence similaritiesBelongs to the alpha/beta interferon family.
  • Cellular localizationSecreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • Alpha 2a interferon antibody
    • IFN alpha 2b antibody
    • IFN-alpha-2 antibody
    • IFNA antibody
    • Ifna2 antibody
    • IFNA2_HUMAN antibody
    • INFA2 antibody
    • Interferon alpha 2 antibody
    • Interferon alpha A antibody
    • Interferon alpha-2 antibody
    • Interferon alpha-A antibody
    • LeIF A antibody
    • LeIFA antibody
    see all

Anti-Interferon alpha 2b antibody [4E10] images

  • Immunoblot of human interferon alpha 2b using monoclonal antibody 4E10 at a dilution of 1:500 (4 µg/ml).
    Lane 1 - interferon alpha 2b
    Lane 2 - prestained standard proteins.

References for Anti-Interferon alpha 2b antibody [4E10] (ab9388)

ab9388 has not yet been referenced specifically in any publications.

Product Wall

Thank you for contacting us. Yes, these are the same lot numbers in all cases. The number after the - just indicates the time it was aliquotted, so if the GRXXXX is the same but the dash is different you can be assured the lot is still the same. Please...

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Thank you for contacting us.

We have used this antibody under reducing and denaturing conditions in western blot. We unfortunately haven't done any comparison studies between native vs reducing conditions. I am sorry we are unable to comment o...

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Thank you for contacting us. Concentrations of the antibodies ab9386 and ab9388 are determined spectrophotometrically by an adsorbance measurement at 280 nm. The absorbance value (A280) is divided by the extiction coefficient of mouse IgG - 1.35. I hop...

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Thank you for contacting Abcam. For both ab9386 and ab9388, we currently sell them in 100ul aliquots, at a concentration of 2mg/ml. From what I can see, there are no plans to change this concentration for future lots of the antibody and since it is a m...

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Thank you for your enquiry. We currently do not have an antibody to human interferon alpha that is biotinylated. We have the following human interferon alpha antibodies: ab9660, ab9386, ab8317, ab9388, and ab5258 (HRP conjugated). If you have any more ...

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To use monoclonal antibodies 9D3 and 4E10 in sandwich ELISA for the detection of huIFN-alpha-2b, 4E10 antibody should be labeled either with horse-radish peroxidase or biotin. Monoclonal antibody 9D3 should be used as a capture antibody (for plate coat...

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