Anti-Interferon alpha 2b antibody [4E10] (HRP) (ab5258)


  • Product nameAnti-Interferon alpha 2b antibody [4E10] (HRP)
    See all Interferon alpha 2b primary antibodies
  • Description
    Mouse monoclonal [4E10] to Interferon alpha 2b (HRP)
  • ConjugationHRP
  • SpecificityRecognizes both human recombinant IFN-a2b and IFN-a2a. Does not react with human recombinant IFN-a5 and IFN-g.
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant full length protein (Human).

  • Positive control
  • General notesRecommended ELISA procedure for human IFN alpha 2b determination: 1. Use 96-well Nunc Maxisorp plates for the assay. Coat microwells with 100 µl/well of antibody 9D3 (ab9386) diluted to 10 µg/ml in coating buffer (0.05 M Na-carbonate, pH 9.5). Incubate overnight at +4°C. 2. Aspirate wells and block with 150 µl/well of blocking buffer (2% BSA in PBS). Incubate at RT for 1 h. 3. Aspirate wells and wash 3 times with PBST (0.1% Tween-20 in PBS). 4. Prepare standard and sample dilutions in PBST. Recommended IFN alpha 2b concentrations: from 100 pg/ml to 100 ng/ml. Add 100 µl of each standard and sample into appropriate wells. Incubate at RT for 2 h. 5. Aspirate and wash 5 times with PBST. 6. Dilute detection antibody 4E10-HRP (ab5258) 1:1000 in PBST. Add 100 µl of diluted detection antibody to each well. Incubate at RT for 1 h. 7. Aspirate and wash 10 times with PBST. 8. Add 100 µl of substrate solution (TMB) to each well. Incubate at RT for 10-20 min. 9. Add 50 µl of stop solution (2 N H2SO4) to each well. Read absorbance at 450 nm.



Our Abpromise guarantee covers the use of ab5258 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
  • Application notesSandwich ELISA: 1/1000.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    This antibody can be used in two-site ELISA for the determination of human IFN-a2b.
    It recognizes a different epitope than monoclonal antibody 9D3 (ab9386).
    We recommend using this antibody (ab5258) as a detection antibody.
  • Target

    • FunctionProduced by macrophages, IFN-alpha have antiviral activities.
    • Sequence similaritiesBelongs to the alpha/beta interferon family.
    • Cellular localizationSecreted.
    • Information by UniProt
    • Database links
    • Alternative names
      • Alpha 2a interferon antibody
      • IFN alpha 2b antibody
      • IFN-alpha-2 antibody
      • IFNA antibody
      • Ifna2 antibody
      • IFNA2_HUMAN antibody
      • INFA2 antibody
      • Interferon alpha 2 antibody
      • Interferon alpha A antibody
      • Interferon alpha-2 antibody
      • Interferon alpha-A antibody
      • LeIF A antibody
      • LeIFA antibody
      see all

    References for Anti-Interferon alpha 2b antibody [4E10] (HRP) (ab5258)

    ab5258 has not yet been referenced specifically in any publications.

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Human Recombinant protein (E coli)
    Loading amount 2 µg
    Specification E coli
    Gel Running Conditions Reduced Denaturing
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C

    Abcam user community

    Verified customer

    Submitted Sep 13 2011