Anti-Interferon alpha antibody (ab24767)

Overview

  • Product nameAnti-Interferon alpha antibody
    See all Interferon alpha primary antibodies
  • Description
    Rabbit polyclonal to Interferon alpha
  • SpecificityRecognizes chicken Interferon alpha (chIFN-alpha). The antibody cross reacts with IFN beta but does not cross react with IFN gamma. This antibody will recognize chicken IFN beta by Western blotting; it does not neutralize its activity in vitro. It does, however, neutralize the activity of IFN alpha, and thus it can be used to distinguish the two bioactivities. Presumably it recognizes an epitope that is only revealed in IFN beta following denaturation.
  • Tested applicationsSuitable for: ELISA, IP, WB, Functional Studiesmore details
  • Species reactivity
    Reacts with: Chicken
  • Immunogen

    Recombinant full length protein (Chicken)

  • General notes


    The classification of mammalian cytokines with antiviral activity, is well documented as being either Type I Interferon (IFNI) or Type II Interferon (IFNII). This is not the case for avian IFNs however, even though IFN was first detected in the chicken. A growing number of functional studies indicate similarities between chicken IFN and mammalian IFNI, revealing the conservation of cysteine residues and the existence of around ten closely related intron-less IFN alpha genes. Further research has shown that chIFN-alpha genes are strongly expressed following viral infection of monocyte-derived macrophage and embryo fibroblasts and that recombinant chicken IFN acts as a potent antiviral agent.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: None
  • Concentration information loading...
  • PurityProtein G purified
  • Primary antibody notesThe classification of mammalian cytokines with antiviral activity, is well documented as being either Type I Interferon (IFNI) or Type II Interferon (IFNII). This is not the case for avian IFNs however, even though IFN was first detected in the chicken. A growing number of functional studies indicate similarities between chicken IFN and mammalian IFNI, revealing the conservation of cysteine residues and the existence of around ten closely related intron-less IFN alpha genes. Further research has shown that chIFN-alpha genes are strongly expressed following viral infection of monocyte-derived macrophage and embryo fibroblasts and that recombinant chicken IFN acts as a potent antiviral agent.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab24767 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent dilution.
IP Use at an assay dependent dilution.
WB Use at an assay dependent dilution. Detects a band of approximately 19 kDa.
Functional Studies Use at an assay dependent dilution.

Target

  • FunctionProduced by macrophages, IFN-alpha have antiviral activities. Interferon stimulates the production of two enzymes: a protein kinase and an oligoadenylate synthetase.
  • Sequence similaritiesBelongs to the alpha/beta interferon family.
  • Cellular localizationSecreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • IFL antibody
    • IFN alpha antibody
    • IFN alpha 1/13 antibody
    • IFN alpha 1b antibody
    • IFN alphaD antibody
    • IFN antibody
    • IFN-alpha-1/13 antibody
    • IFNA@ antibody
    • IFNA1 antibody
    • IFNA1_HUMAN antibody
    • IFNA13 antibody
    • Interferon alpha 1 antibody
    • Interferon alpha-1/13 antibody
    • Interferon alpha-D antibody
    • Interferon alpha1 antibody
    • Interferon, alpha 13 antibody
    • LeIF D antibody
    see all

References for Anti-Interferon alpha antibody (ab24767)

ab24767 has not yet been referenced specifically in any publications.

Product Wall

Thank you for contacting us.  We do not test the concentration of the specific antibody in our protein G affinity purified antibodies. The 0.5 mg/mL concentration would correspond to total Ig. However, we expect >90% of the antibody to be specific in t...

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Thank you for your inquiry. Generally with affinity purified IgG, we expect >90% specific antibody. We do not measure the specific IgG concentration for these affinity purified products, just total IgG. I hope this information is helpful.  Plea...

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Thank you for your enquiry. The situation with this antibody is that while it will recognize chicken interferon beta by Western blotting, it does not neutralize its activity in vitro. It does, however, neutralize the activity of interferon alpha, and t...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"