Anti-Interferon alpha 1 antibody (ab24767)
Key features and details
- Rabbit polyclonal to Interferon alpha 1
- Suitable for: ELISA, IP, WB, Functional Studies
- Reacts with: Chicken
- Isotype: IgG
Overview
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Product name
Anti-Interferon alpha 1 antibody
See all Interferon alpha 1 primary antibodies -
Description
Rabbit polyclonal to Interferon alpha 1 -
Host species
Rabbit -
Specificity
Recognizes chicken Interferon alpha 1 (chIFN-alpha). The antibody cross reacts with IFN beta but does not cross react with IFN gamma. This antibody will recognize chicken IFN beta by Western blotting; it does not neutralize its activity in vitro. It does, however, neutralize the activity of IFN alpha, and thus it can be used to distinguish the two bioactivities. Presumably it recognizes an epitope that is only revealed in IFN beta following denaturation. -
Tested applications
Suitable for: ELISA, IP, WB, Functional Studiesmore details -
Species reactivity
Reacts with: Chicken -
Immunogen
Recombinant full length protein corresponding to Chicken Interferon alpha 1.
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General notes
The classification of mammalian cytokines with antiviral activity, is well documented as being either Type I Interferon (IFNI) or Type II Interferon (IFNII). This is not the case for avian IFNs however, even though IFN was first detected in the chicken. A growing number of functional studies indicate similarities between chicken IFN and mammalian IFNI, revealing the conservation of cysteine residues and the existence of around ten closely related intron-less IFN alpha genes. Further research has shown that chIFN-alpha genes are strongly expressed following viral infection of monocyte-derived macrophage and embryo fibroblasts and that recombinant chicken IFN acts as a potent antiviral agent.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Concentration information loading...
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Purity
Protein G purified -
Primary antibody notes
The classification of mammalian cytokines with antiviral activity, is well documented as being either Type I Interferon (IFNI) or Type II Interferon (IFNII). This is not the case for avian IFNs however, even though IFN was first detected in the chicken. A growing number of functional studies indicate similarities between chicken IFN and mammalian IFNI, revealing the conservation of cysteine residues and the existence of around ten closely related intron-less IFN alpha genes. Further research has shown that chIFN-alpha genes are strongly expressed following viral infection of monocyte-derived macrophage and embryo fibroblasts and that recombinant chicken IFN acts as a potent antiviral agent. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab24767 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ELISA |
Use at an assay dependent concentration.
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IP |
Use at an assay dependent concentration.
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WB |
Use at an assay dependent concentration.
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Functional Studies |
Use at an assay dependent concentration.
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Notes |
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ELISA
Use at an assay dependent concentration. |
IP
Use at an assay dependent concentration. |
WB
Use at an assay dependent concentration. |
Functional Studies
Use at an assay dependent concentration. |
Target
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Function
Produced by macrophages, IFN-alpha have antiviral activities. Interferon stimulates the production of two enzymes: a protein kinase and an oligoadenylate synthetase. -
Sequence similarities
Belongs to the alpha/beta interferon family. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 396398 Chicken
- SwissProt: P42165 Chicken
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Alternative names
- IFL antibody
- IFN alpha antibody
- IFN alpha 1/13 antibody
see all
Protocols
Datasheets and documents
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Datasheet download
References (0)
ab24767 has not yet been referenced specifically in any publications.