Anti-Interferon alpha antibody [AE3] (ab10077)

Overview

  • Product name
    Anti-Interferon alpha antibody [AE3]
    See all Interferon alpha primary antibodies
  • Description
    Mouse monoclonal [AE3] to Interferon alpha
  • Tested applications
    Suitable for: WB, Sandwich ELISA, ELISAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant full length protein (Human).

  • General notes
    Concentration varies from lot to lot and can be provided on request.

    Abcam is committed to meeting high standards of ethical manufacturing and as such, we will be discontinuing this product, which has been generated by the ascites method, within the next year. We are sorry for any inconvenience this may cause. If you would like help finding an alternative product, please do not hesitate to contact our scientific support team.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.1% Sodium Azide
    Constituents: PBS, pH 7.2
  • Concentration information loading...
  • Purity
    Protein A purified
  • Purification notes
    Purity tested by electrophoresis.
  • Clonality
    Monoclonal
  • Clone number
    AE3
  • Myeloma
    x63-Ag8.653
  • Isotype
    IgG2a
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab10077 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent dilution. Predicted molecular weight: 23 kDa.
Sandwich ELISA Use a concentration of 5 µg/ml. Can be paired for Sandwich ELISA with Mouse monoclonal [AB5] to Interferon alpha (HRP) (ab10078). Can be used as Capture antibody with recommended pair. Detection limit 5pg/ml.
ELISA Use at an assay dependent dilution.

Target

  • Function
    Produced by macrophages, IFN-alpha have antiviral activities. Interferon stimulates the production of two enzymes: a protein kinase and an oligoadenylate synthetase.
  • Sequence similarities
    Belongs to the alpha/beta interferon family.
  • Cellular localization
    Secreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • IFL antibody
    • IFN alpha antibody
    • IFN alpha 1/13 antibody
    • IFN alpha 1b antibody
    • IFN alphaD antibody
    • IFN antibody
    • IFN-alpha-1/13 antibody
    • IFNA@ antibody
    • IFNA1 antibody
    • IFNA1_HUMAN antibody
    • IFNA13 antibody
    • Interferon alpha 1 antibody
    • Interferon alpha-1/13 antibody
    • Interferon alpha-D antibody
    • Interferon alpha1 antibody
    • Interferon, alpha 13 antibody
    • LeIF D antibody
    see all

References

ab10077 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Western blot
Loading amount
30 µg
Gel Running Conditions
Reduced Denaturing
Sample
Human Cell lysate - whole cell (macrophage)
Specification
macrophage
Treatment
0.1 ug/ml LPS 24 h
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Dec 22 2014

Thank you for contacting us. We have indeed in our catalog 5 unconjugated anti-interferon alpha 1 antibodies tested and guaranteed to work in Western Blot and Human samples. Please note that, unfortunately, import restrictions make the ...

Read More

The sensitivity of pair ab10078 & ab10077 depends on the protocol and especially the detection method. With HRP conjugated detection antibody we have reached detection limit ~150 pg/ml, but with biotin-conjugated detection antibody and ExtrAvidin Perox...

Read More

Thank you for your reply. I'm very sorry to hear your recent experiment with the freshly coated plate did not work either. Looking back at your protocol one thing I noticed was you mentiond "Stored at 4 degrees in the same condition it arrived in". Wa...

Read More

I received today the following information from the laboratory: In principle the customer’s protocol seems to be fine. The differences are that we are using biotin-conjugated MAb AB5 for detection and also we have never tried the drying step and sto...

Read More

Thank you for your enquiry. The concentration of the product lot95725 is 7.5 mg/ml. Please do not hesitate to contact us if you require further assistance,

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up