Anti-Interferon alpha antibody [AE3] (ab10077)


  • Product nameAnti-Interferon alpha antibody [AE3]
    See all Interferon alpha primary antibodies
  • Description
    Mouse monoclonal [AE3] to Interferon alpha
  • Tested applicationsSuitable for: WB, Sandwich ELISA, ELISAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant full length protein (Human).

  • General notesConcentration varies from lot to lot and can be provided on request.


  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: 0.1% Sodium Azide
    Constituents: PBS, pH 7.2
  • Concentration information loading...
  • PurityProtein A purified
  • Purification notesPurity tested by electrophoresis.
  • ClonalityMonoclonal
  • Clone numberAE3
  • Myelomax63-Ag8.653
  • IsotypeIgG2a
  • Research areas


Our Abpromise guarantee covers the use of ab10077 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent dilution. Predicted molecular weight: 23 kDa.
Sandwich ELISA Use a concentration of 5 µg/ml. Can be paired for Sandwich ELISA with Mouse monoclonal [AB5] to Interferon alpha (HRP) (ab10078). Can be used as Capture antibody with recommended pair. Detection limit 5pg/ml.
ELISA Use at an assay dependent dilution.


  • FunctionProduced by macrophages, IFN-alpha have antiviral activities. Interferon stimulates the production of two enzymes: a protein kinase and an oligoadenylate synthetase.
  • Sequence similaritiesBelongs to the alpha/beta interferon family.
  • Cellular localizationSecreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • IFL antibody
    • IFN alpha antibody
    • IFN alpha 1/13 antibody
    • IFN alpha 1b antibody
    • IFN alphaD antibody
    • IFN antibody
    • IFN-alpha-1/13 antibody
    • IFNA@ antibody
    • IFNA1 antibody
    • IFNA1_HUMAN antibody
    • IFNA13 antibody
    • Interferon alpha 1 antibody
    • Interferon alpha-1/13 antibody
    • Interferon alpha-D antibody
    • Interferon alpha1 antibody
    • Interferon, alpha 13 antibody
    • LeIF D antibody
    see all

References for Anti-Interferon alpha antibody [AE3] (ab10077)

ab10077 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Loading amount 30 µg
Gel Running Conditions Reduced Denaturing
Sample Human Cell lysate - whole cell (macrophage)
Specification macrophage
Treatment 0.1 ug/ml LPS 24 h
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Dec 22 2014

Thank you for contacting us. We have indeed in our catalog 5 unconjugated anti-interferon alpha 1 antibodies tested and guaranteed to work in Western Blot and Human samples. Please note that, unfortunately, import restrictions make the ...

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The sensitivity of pair ab10078 & ab10077 depends on the protocol and especially the detection method. With HRP conjugated detection antibody we have reached detection limit ~150 pg/ml, but with biotin-conjugated detection antibody and ExtrAvidin Perox...

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Thank you for your reply. I'm very sorry to hear your recent experiment with the freshly coated plate did not work either. Looking back at your protocol one thing I noticed was you mentiond "Stored at 4 degrees in the same condition it arrived in". Wa...

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I received today the following information from the laboratory: In principle the customer’s protocol seems to be fine. The differences are that we are using biotin-conjugated MAb AB5 for detection and also we have never tried the drying step and sto...

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Thank you for your enquiry. The concentration of the product lot95725 is 7.5 mg/ml. Please do not hesitate to contact us if you require further assistance,