Overview

  • Product nameAnti-Interferon alpha antibody [F18]
    See all Interferon alpha primary antibodies
  • Description
    Rat monoclonal [F18] to Interferon alpha
  • SpecificityThis monoclonal antibody binds and neutralizes both natural and recombinant mouse alpha Interferon. This antibody does not cross-react with Human Interferon, Mouse Interferon beta or Mouse Interferon gamma.
  • Tested applicationsSuitable for: Flow Cyt, Neutralising, Other, Inhibition Assaymore details
  • Species reactivity
    Reacts with: Mouse
    Does not react with: Human
  • Positive control
    • Plasmacytoid dendritic cells.
  • General notes


    With the availability of monoclonal antibodies directed against alpha Interferon, it is possible to interpret results obtained from crude materials containing both alpha and beta Interferon. The difficulties in studying in vitro and in vivo effects of "type 1" Interferons arise from the fact that both alpha and beta Interferons are produced in response to the same stimuli and also seem to act via the same receptor. These Interferon activities can only be distinguished from one another by use of specific neutralizing antibodies.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPBS with 0.1% sodium azide
  • Concentration information loading...
  • PurityIgG fraction
  • Purification notes0.2 µm filtered antibody solution.
  • Primary antibody notesWith the availability of monoclonal antibodies directed against alpha Interferon, it is possible to interpret results obtained from crude materials containing both alpha and beta Interferon. The difficulties in studying in vitro and in vivo effects of "type 1" Interferons arise from the fact that both alpha and beta Interferons are produced in response to the same stimuli and also seem to act via the same receptor. These Interferon activities can only be distinguished from one another by use of specific neutralizing antibodies.
  • ClonalityMonoclonal
  • Clone numberF18
  • IsotypeIgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab7373 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use at an assay dependent concentration. PubMed: 21172865
Neutralising Use at an assay dependent concentration.
Other Use at an assay dependent concentration.
AP Use at an assay dependent concentration.
Inhibition Assay Use at an assay dependent concentration. Useful for inhiiton in biological assays and for affinity pruification.

Target

  • FunctionProduced by macrophages, IFN-alpha have antiviral activities. Interferon stimulates the production of two enzymes: a protein kinase and an oligoadenylate synthetase.
  • Sequence similaritiesBelongs to the alpha/beta interferon family.
  • Cellular localizationSecreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • IFL antibody
    • IFN alpha antibody
    • IFN alpha 1/13 antibody
    • IFN alpha 1b antibody
    • IFN alphaD antibody
    • IFN antibody
    • IFN-alpha-1/13 antibody
    • IFNA@ antibody
    • IFNA1 antibody
    • IFNA1_HUMAN antibody
    • IFNA13 antibody
    • Interferon alpha 1 antibody
    • Interferon alpha-1/13 antibody
    • Interferon alpha-D antibody
    • Interferon alpha1 antibody
    • Interferon, alpha 13 antibody
    • LeIF D antibody
    see all

References for Anti-Interferon alpha antibody [F18] (ab7373)

This product has been referenced in:
  • Björck P  et al. Plasmacytoid dendritic cell dichotomy: identification of IFN-a producing cells as a phenotypically and functionally distinct subset. J Immunol 186:1477-85 (2011). Flow Cyt ; Mouse . Read more (PubMed: 21172865) »
  • Joshi VD  et al. A role for Stat1 in the regulation of lipopolysaccharide-induced interleukin-1beta expression. J Interferon Cytokine Res 26:739-47 (2006). Read more (PubMed: 17032168) »

See all 2 Publications for this product

Product Wall

Thank you for your enquiry.

I am sorry to confirm that ab27715 is sourced externally and regrettably we are not able to provide the immunogen sequence. The source considers this to be proprietary information on this occasion. However, they h...

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Thank you for your inquiry.

Yes, I can confirm that GRxx-2 and xx are both the same lot. These are just two different names for the same.I am sorry for this confusion and willforward this feedback.

Indeed, we added the GR lot number...

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Thank you for your message. If you have not already done so, you can place an order by calling us on +44(0)1223 696000 or email orders@abcam.com. Good luck with your research!

The antibody has been raised against purified natural L-cell IFN-alpha. The antibody reacts with alpha-1, alpha-2, alpha-4, alpha-5 (P), alpha-6 (T) and alpha-A subtypes.

Thank you for your enquiry. Ab7373 is only available in the form described on the online datasheet and we cannot do specialized orders.

Thank you for your enquiry. Ab7373 has so far only been tested for application in Immunoprecipitation, Affinity purification, Immunoassay, and Inhibition Assay. Our mouse monoclonal Interferon alpha 2b antibodies - ab9386, ab9388, and ab5258 - all have...

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We regret to inform you that we do not have any further information than the answer we have send to you.

Any rat IgG1 monoclonal antibody which doesn't bind to IFN-alpha can be useful as control.

The antibody recognizes natural IFN-alpha from L-cells and several recombinant IFN-alpha subtypes. Neutralization of alpha-1, alpha-2, alpha-4, alpha-5 (P), alpha-6 (T) and alpha-A subtypes is possible.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"