Overview

  • Product name
    Anti-Interferon beta antibody (Biotin)
    See all Interferon beta primary antibodies
  • Description
    Rabbit polyclonal to Interferon beta (Biotin)
  • Host species
    Rabbit
  • Conjugation
    Biotin
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Highly pure (>98%) recombinant human Interferon beta

  • Positive control
    • This antibody gave a positive signal in human liver, spleen and kidney tissue lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab84258 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 27 kDa (predicted molecular weight: 22 kDa).
IHC-P Use a concentration of 0.75 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF Use a concentration of 5 µg/ml.

Target

  • Function
    Has antiviral, antibacterial and anticancer activities.
  • Sequence similarities
    Belongs to the alpha/beta interferon family.
  • Cellular localization
    Secreted.
  • Information by UniProt
  • Database links
    • Alternative names
      • beta-interferon antibody
      • Fibroblast interferon antibody
      • IFB antibody
      • IFF antibody
      • IFN beta antibody
      • IFN-beta antibody
      • IFNB 1 antibody
      • IFNB antibody
      • IFNB_HUMAN antibody
      • IFNB1 antibody
      • Interferon beta 1 fibroblast antibody
      • Interferon beta antibody
      • Interferon beta precursor antibody
      • MGC96956 antibody
      see all

    Images

    • Immunocytochemistry/ Immunofluorescence - Anti-Interferon beta antibody (Biotin) (ab84258)
      Immunocytochemistry/ Immunofluorescence - Anti-Interferon beta antibody (Biotin) (ab84258)
      ICC/IF image of ab84258 stained HepG2 cells. The cells were 4% formaldehyde (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab84258, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

    • Western blot - Anti-Interferon beta antibody (Biotin) (ab84258)
      Western blot - Anti-Interferon beta antibody (Biotin) (ab84258)
      All lanes : Anti-Interferon beta antibody (Biotin) (ab84258) at 1 µg/ml

      Lane 1 : Human liver tissue lysate - total protein (ab29889)
      Lane 2 : Human spleen tissue lysate - total protein (ab29699)
      Lane 3 : Human kidney tissue lysate - total protein (ab30203)

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

      Developed using the ECL technique.

      Performed under reducing conditions.

      Predicted band size: 22 kDa
      Observed band size: 27 kDa (why is the actual band size different from the predicted?)
      Additional bands at: 100 kDa, 46 kDa, 55 kDa. We are unsure as to the identity of these extra bands.


      Exposure time: 16 minutes


      The Interferon Beta protein sequence contains both a phosphorylation and a glycosylation site (Swissprot). Post-translation modifications could account for the migration of the band at a higher molecular weight than predicted.
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Interferon beta antibody (Biotin) (ab84258)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Interferon beta antibody (Biotin) (ab84258)
      Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human normal placenta, staining Interferon beta with ab84258 at 0.75 µg/ml. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 6.0 sodium citrate buffer is recommended.

    References

    This product has been referenced in:
    • Liu Y  et al. Suppression of mRNA Nanoparticle Transfection in Human Fibroblasts by Selected Interferon Inhibiting Small Molecule Compounds. Biomolecules 7:N/A (2017). Read more (PubMed: 28758979) »
    • Price JV  et al. Protein microarray analysis reveals BAFF-binding autoantibodies in systemic lupus erythematosus. J Clin Invest 123:5135-45 (2013). Read more (PubMed: 24270423) »

    See all 2 Publications for this product

    Publishing research using ab84258? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

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    5 reviews or Q&A

    10
    Votes: Highest First

    I got this Discount Code from you a couple months ago or so. And so I
    submitted an AbReview trying to follow all the instructions, so I can get
    myself a free primary antibody as mentioned below. Well so today I
    thought I would order my f...

    Read More

    Thank you for contacting Abcam.
    I have gone ahead and added the discount code in the additional notes section of the Abreview you submitted. You can now go ahead to use the discount code to receive your free antibody.
    If there is anything els...

    Read More

    That sounds like a pretty good offer but my biggest concern is that since we don't know if it works, and if I try it but I don't see any signal in my cells, I won't know if that's because (a) it doesn't work for IF or because (b) my cells aren't mak...

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    Thank you for your reply.

    I do understand your concerns. It may be that you would want to run a western blot with this product to ensure that it is picking up IFN from your cells, it would them be possible to run a purified protein as control...

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    Anti-Interferon beta antibody (Biotin) (ab84258). I noticed the website says it reacts with human IFN. Is there any reason why it shouldn't work to detect other species of IFN as well? I want to do immunofluorescence microscopy for mouse IFN in hepatoc...

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    Thank you very much for your interest in ab84258.

    Unfortunately the sequence homology between he human and mouse forms of IFNB is very low (47%). We would not be confident that this product would work in mouse.

    We do have another pro...

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    I'm very intereating in products of ab6979 and ab84258 of your company. I want use ab6979 as capture antibody in in a sandwich ELISA for analysis of human IFNb, and ab84258 as detection antibody. But I can't find the suitable range of concentrations ab...

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    Thank you for contacting us.

    I think these two antibodies will work together in a sandwich ELISA, but we have not tested them together. You will need to test a few different dilutions to achieve the optimal signal with the least background. ...

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    Is the biotin on the N or C terminal?

    Thank you for contacting Abcam. I have heard back from the lab and they have told me that the biotin is covalently coupled to primary amines (lysines) of the immunoglobulin and not to either the N or C terminal. If there is anything else I can help you...

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