Anti-Interferon beta antibody (Biotin) (ab84258)
- Datasheet
- Specific References (1)
- Protocols
Overview
- Product nameAnti-Interferon beta antibody (Biotin)
See all Interferon beta primary antibodies - DescriptionRabbit polyclonal to Interferon beta (Biotin)
- ConjugationBiotin
- Tested applicationsSuitable for: ELISA, WB, IHC-P, ICC/IFmore details
- Species reactivityReacts with: Human
- Immunogen
Highly pure (>98%) recombinant human Interferon beta
- Positive control
- This antibody gave a positive signal in human liver, spleen and kidney tissue lysates.
Properties
- FormLyophilised:Centrifuge vial prior to opening. Reconstitute in sterile PBS containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml.
- Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
- Storage bufferPreservative: None
Constituents: PBS, pH 7.2 -
Concentration information loading... - PurityProtein A purified
- Purification notesab84258 is sterile filtered.
- ClonalityPolyclonal
- IsotypeIgG
- Research areas
Associated products
- Compatible Secondaries
Applications
Our Abpromise guarantee covers the use of ab84258 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ELISA | Use a concentration of 0.25 - 1 µg/ml. Allows the detection of at least 0.2 - 0.4 ng/well of recombinant human Interferon beta. | |
| WB | Use a concentration of 1 µg/ml. Detects a band of approximately 27 kDa (predicted molecular weight: 22 kDa). | |
| IHC-P | Use a concentration of 0.75 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. | |
| ICC/IF | Use a concentration of 5 µg/ml. |
Target
- FunctionHas antiviral, antibacterial and anticancer activities.
- Sequence similaritiesBelongs to the alpha/beta interferon family.
- Cellular localizationSecreted.
- Information by UniProt
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Database links
- Entrez Gene: 3456 Human
- Omim: 147640 Human
- SwissProt: P01574 Human
- Unigene: 93177 Human
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Alternative names
- beta-interferon antibody
- Fibroblast interferon antibody
- IFB antibody
see all
Anti-Interferon beta antibody (Biotin) images
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Immunocytochemistry/ Immunofluorescence - Anti-Interferon beta antibody (Biotin) (ab84258)ICC/IF image of ab84258 stained HepG2 cells. The cells were 4% formaldehyde (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab84258, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. -
Western blot - Anti-Interferon beta antibody (Biotin) (ab84258)All lanes : Anti-Interferon beta antibody (Biotin) (ab84258) at 1 µg/ml
Lane 1 :Human liver tissue lysate - total protein (ab29889)
Lane 2 :Human spleen tissue lysate - total protein (ab29699)
Lane 3 :Human kidney tissue lysate - total protein (ab30203)
Lysates/proteins at 10 µg per lane.
Secondary
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 22 kDa
Observed band size : 27 kDa (why is the actual band size different from the predicted?)
Additional bands at : 100 kDa,46 kDa,55 kDa. We are unsure as to the identity of these extra bands.
Exposure time : 16 minutes -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Interferon beta antibody (Biotin) (ab84258)Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human normal placenta, staining Interferon beta with ab84258 at 0.75 µg/ml. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 6.0 sodium citrate buffer is recommended.
References for Anti-Interferon beta antibody (Biotin) (ab84258)
This product has been referenced in:
- Price JV et al. Protein microarray analysis reveals BAFF-binding autoantibodies in systemic lupus erythematosus. J Clin Invest 123:5135-45 (2013). Read more (PubMed: 24270423) »
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