Anti-Interferon gamma antibody [4S.B3] (Phycoerythrin) (ab178447)

Overview

  • Product name
    Anti-Interferon gamma antibody [4S.B3] (Phycoerythrin)
    See all Interferon gamma primary antibodies
  • Description
    Mouse monoclonal [4S.B3] to Interferon gamma (Phycoerythrin)
  • Host species
    Mouse
  • Conjugation
    Phycoerythrin. Ex: 488nm, Em: 575nm
  • Specificity
    Binds both glycosylated and non-glycosylated protein.
  • Tested applications
    Suitable for: Flow Cytmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Non human primates
  • Immunogen

    Full length native protein (purified) corresponding to Human Interferon gamma aa 1-166. Interferon gamma derived from Human leukocytes.
    Sequence:

    MKYTSYILAF QLCIVLGSLG CYCQDPYVKE AENLKKYFNA GHSDVADNGT LFLGILKNWK EESDRKIMQS QIVSFYFKLF KNFKDDQSIQ KSVETIKEDM NVKFFNSNKK KRDDFEKLTN YSVTDLNVQR KAIHELIQVM AELSPAAKTG KRKRSQMLFR GRRASQ


    Database link: P01579

Properties

Applications

Our Abpromise guarantee covers the use of ab178447 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 10µl for 106 cells.

or 100 μl of whole blood.

 

 

 

 

ab91357 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

 

Target

  • Function
    Produced by lymphocytes activated by specific antigens or mitogens. IFN-gamma, in addition to having antiviral activity, has important immunoregulatory functions. It is a potent activator of macrophages, it has antiproliferative effects on transformed cells and it can potentiate the antiviral and antitumor effects of the type I interferons.
  • Tissue specificity
    Released primarily from activated T lymphocytes.
  • Involvement in disease
    In Caucasians, genetic variation in IFNG is associated with the risk of aplastic anemia (AA) [MIM:609135]. AA is a rare disease in which the reduction of the circulating blood cells results from damage to the stem cell pool in bone marrow. In most patients, the stem cell lesion is caused by an autoimmune attack. T-lymphocytes, activated by an endogenous or exogenous, and most often unknown antigenic stimulus, secrete cytokines, including IFN-gamma, which would in turn be able to suppress hematopoiesis.
  • Sequence similarities
    Belongs to the type II (or gamma) interferon family.
  • Post-translational
    modifications
    Proteolytic processing produces C-terminal heterogeneity, with proteins ending alternatively at Gly-150, Met-157 or Gly-161.
  • Cellular localization
    Secreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • IFG antibody
    • IFI antibody
    • IFN gamma antibody
    • IFN, immune antibody
    • IFN-gamma antibody
    • IFNG antibody
    • IFNG_HUMAN antibody
    • Immune interferon antibody
    • Interferon gamma antibody
    see all

Images

  • Flow Cytometry analysis of PHA-activated human peripheral blood cells labeling Interferon gamma with Anti-Interferon gamma antibody [4S.B3] (Phycoerythrin) (ab178447).
  • Intracellular flow cytometry analysis of Interferon gamma expression in PHA-activated human PBMC with Anti-Interferon gamma antibody [4S.B3] (Phycoerythrin) (ab178447).

References

ab178447 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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