Recombinant full length protein corresponding to Human Interferon gamma. Mature Interferon gamma is from aa 24-161 of SwissProt ID Q14609. aa 1-23 represents the signal peptide.
MQDPYVKEAENLKKYFNAGHSDVADNGTLFLGILKNWKEESDRKIMQSQI VSFYFKLFKN FKDDQSIQKS VETIKEDMNV KFFNSNKKKRDDFEKLTNYSVTDLNVQRKAIHELIQVMAELSPAAKTGKR KRSQMLFQGR RASQ
Our Abpromise guarantee covers the use of ab9657 in the following tested applications.
|WB||Use at an assay dependent concentration. To detect hIFN-gamma by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIFN-gamma is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.|
|ELISA||Use at an assay dependent concentration. Can be paired for ELISA with Mouse monoclonal to Interferon gamma (ab9658).
To detect IFN-gamma by sandwich ELISA (using 100µl/well antibody solution) a concentration of at least 0.5µg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hIFN-gamma.
|Neutralising||Use at an assay dependent concentration. To yield one-half maximal inhibition [ND50] of the biological activity of hIFN-gamma (50 pg/ml), a concentration of 0.04 - 0.06 ng/ml of this antibody is required.|
|IHC-P||Use a concentration of 0.25 - 0.5 µg/ml.|
To detect Human IFN-γ by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with a compatible secondary detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Human IFN-γ.