Overview

  • Product nameAnti-Interferon gamma antibody
    See all Interferon gamma primary antibodies
  • Description
    Rabbit polyclonal to Interferon gamma
  • Tested applicationsSuitable for: Sandwich ELISA, WB, ELISA, Neutralisingmore details
  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    Highly pure (>98%) recombinant mIFN-g (mouse Interferon-gamma).

Properties

Applications

Our Abpromise guarantee covers the use of ab9918 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use a concentration of 0.5 - 2 µg/ml. Can be paired for Sandwich ELISA with Rabbit polyclonal to Interferon gamma (Biotin) (ab83136). This antigen affinity purified antibody, in conjunction with recommended pair as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mIFN-gamma. Can be used as capture antibody when paired with ab83136.
WB Use a concentration of 0.1 - 0.2 µg/ml.
ELISA Use a concentration of 0.5 µg/ml. Allows the detection of 0.2 - 0.4 ng/well of recombinant mIFN-g.
Neutralising Use a concentration of 0.004 - 0.006 µg/ml. for one-half maximal inhibition [ND50] of the biological activity of mIFN-g (50 pg/ml).

Target

  • FunctionProduced by lymphocytes activated by specific antigens or mitogens. IFN-gamma, in addition to having antiviral activity, has important immunoregulatory functions. It is a potent activator of macrophages, it has antiproliferative effects on transformed cells and it can potentiate the antiviral and antitumor effects of the type I interferons.
  • Tissue specificityReleased primarily from activated T lymphocytes.
  • Involvement in diseaseIn Caucasians, genetic variation in IFNG is associated with the risk of aplastic anemia (AA) [MIM:609135]. AA is a rare disease in which the reduction of the circulating blood cells results from damage to the stem cell pool in bone marrow. In most patients, the stem cell lesion is caused by an autoimmune attack. T-lymphocytes, activated by an endogenous or exogenous, and most often unknown antigenic stimulus, secrete cytokines, including IFN-gamma, which would in turn be able to suppress hematopoiesis.
  • Sequence similaritiesBelongs to the type II (or gamma) interferon family.
  • Post-translational
    modifications
    Proteolytic processing produces C-terminal heterogeneity, with proteins ending alternatively at Gly-150, Met-157 or Gly-161.
  • Cellular localizationSecreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • IFG antibody
    • IFI antibody
    • IFN gamma antibody
    • IFN, immune antibody
    • IFN-gamma antibody
    • IFNG antibody
    • IFNG_HUMAN antibody
    • Immune interferon antibody
    • Interferon gamma antibody
    see all

References for Anti-Interferon gamma antibody (ab9918)

This product has been referenced in:
  • Mastronardi FG  et al. Synergy between paclitaxel plus an exogenous methyl donor in the suppression of murine demyelinating diseases. Mult Scler 13:596-609 (2007). Read more (PubMed: 17548438) »

See 1 Publication for this product

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I have been in contact with the lab, I can confirm that ab9918 has already been reconstituted in 200ul sterile water. Since this antibody was lyophilised in PBS, this means that upon the addition of the wate...

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There may be some antibodies where I might have suggested the human tissues or cells. I apologize for this. I have included the positive control information irrespective of species so similar mouse tissues sections ca...

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The positive control for ab36392 is IL2 activatedcultured NK cells.

ab9918; human tonsil sections, human cervical squamous cell carcinoma section

ab34674; human artery or colon tissue sections
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It does look like we changed the storage instructions of ab2557. Since this product was tested in a guaranteed species and technique and she wasn't seeing any staining, we could offer a free of charge replacement ord...

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Thank you forhaving your customercomplete our questionnaire. I am sorry to hear that these antibodies are not providing satisfactory results for them. The details provided will enable us to investigate this case and will provide us with vital informati...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"