Anti-Interferon gamma antibody (Biotin) (ab83136)

Overview

  • Product nameAnti-Interferon gamma antibody (Biotin)
    See all Interferon gamma primary antibodies
  • Description
    Rabbit polyclonal to Interferon gamma (Biotin)
  • ConjugationBiotin
  • Tested applicationsSuitable for: ELISA, Sandwich ELISA, WBmore details
  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    Highly pure (>98%) recombinant mouse Interferon gamma.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab83136 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use a concentration of 0.25 - 1 µg/ml. Allows the detection of at least 0.2 - 0.4 ng/well of recombinant murine Interferon gamma.
Sandwich ELISA Use a concentration of 0.25 - 1 µg/ml. Can be paired for Sandwich ELISA with Rabbit polyclonal to Interferon gamma (ab9918). This biotinylated polyclonal antibody, in conjunction with recommended pair as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant mIFN-gamma. Can be used as detection antibody when paired with ab9918.
WB Use a concentration of 0.1 - 0.2 µg/ml. Predicted molecular weight: 18 kDa. The detection limit for recombinant murine Interferon gamma is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Target

  • FunctionProduced by lymphocytes activated by specific antigens or mitogens. IFN-gamma, in addition to having antiviral activity, has important immunoregulatory functions. It is a potent activator of macrophages, it has antiproliferative effects on transformed cells and it can potentiate the antiviral and antitumor effects of the type I interferons.
  • Tissue specificityReleased primarily from activated T lymphocytes.
  • Involvement in diseaseIn Caucasians, genetic variation in IFNG is associated with the risk of aplastic anemia (AA) [MIM:609135]. AA is a rare disease in which the reduction of the circulating blood cells results from damage to the stem cell pool in bone marrow. In most patients, the stem cell lesion is caused by an autoimmune attack. T-lymphocytes, activated by an endogenous or exogenous, and most often unknown antigenic stimulus, secrete cytokines, including IFN-gamma, which would in turn be able to suppress hematopoiesis.
  • Sequence similaritiesBelongs to the type II (or gamma) interferon family.
  • Post-translational
    modifications
    Proteolytic processing produces C-terminal heterogeneity, with proteins ending alternatively at Gly-150, Met-157 or Gly-161.
  • Cellular localizationSecreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • IFG antibody
    • IFI antibody
    • IFN gamma antibody
    • IFN, immune antibody
    • IFN-gamma antibody
    • IFNG antibody
    • IFNG_HUMAN antibody
    • Immune interferon antibody
    • Interferon gamma antibody
    see all

References for Anti-Interferon gamma antibody (Biotin) (ab83136)

ab83136 has not yet been referenced specifically in any publications.

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