Anti-Interferon gamma antibody [D9D10] (ab11412)

Overview

  • Product nameAnti-Interferon gamma antibody [D9D10]
    See all Interferon gamma primary antibodies
  • Description
    Mouse monoclonal [D9D10] to Interferon gamma
  • Specificityab11412 recognises both natural and recombinant human interferon gamma, binding only to the dimeric (functional) form. Clone D9D10 has been reported to neutralise the activity of natural and recombinant IFN gamma. The removal of sodium azide is recommended for functional assays.
  • Tested applicationsSuitable for: Blocking, Flow Cyt, ELISA, WBmore details
  • Species reactivity
    Reacts with: Human, Baboon
  • Immunogen

    Full length native protein (purified) (Human).

  • General notesThis product should be stored undiluted. Storage in frost free freezers is not recommended. Should this product contain a precipitate we recommend microcentrifugation before use.

Properties

Applications

Our Abpromise guarantee covers the use of ab11412 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Blocking Use a concentration of 1 µg/ml.
Flow Cyt 1/10 - 1/100.

Use 10 µl of the suggested working dilution to label 1 x 106 cells in 100 µl.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

ELISA Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Use under non reducing condition. Predicted molecular weight: 19.9 kDa.

Use under non reducing conditions.

Target

  • FunctionProduced by lymphocytes activated by specific antigens or mitogens. IFN-gamma, in addition to having antiviral activity, has important immunoregulatory functions. It is a potent activator of macrophages, it has antiproliferative effects on transformed cells and it can potentiate the antiviral and antitumor effects of the type I interferons.
  • Tissue specificityReleased primarily from activated T lymphocytes.
  • Involvement in diseaseIn Caucasians, genetic variation in IFNG is associated with the risk of aplastic anemia (AA) [MIM:609135]. AA is a rare disease in which the reduction of the circulating blood cells results from damage to the stem cell pool in bone marrow. In most patients, the stem cell lesion is caused by an autoimmune attack. T-lymphocytes, activated by an endogenous or exogenous, and most often unknown antigenic stimulus, secrete cytokines, including IFN-gamma, which would in turn be able to suppress hematopoiesis.
  • Sequence similaritiesBelongs to the type II (or gamma) interferon family.
  • Post-translational
    modifications
    Proteolytic processing produces C-terminal heterogeneity, with proteins ending alternatively at Gly-150, Met-157 or Gly-161.
  • Cellular localizationSecreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • IFG antibody
    • IFI antibody
    • IFN gamma antibody
    • IFN, immune antibody
    • IFN-gamma antibody
    • IFNG antibody
    • IFNG_HUMAN antibody
    • Immune interferon antibody
    • Interferon gamma antibody
    see all

References for Anti-Interferon gamma antibody [D9D10] (ab11412)

ab11412 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Blocking
Sample Human Cultured Cells (Block of STAT1 phosphorilation in IM9 myeloma cell)
Specification Block of STAT1 phosphorilation in IM9 myeloma cell
Username

Dr. Roberto Bellucci

Verified customer

Submitted Jul 18 2012

Thank you for your reply.  After reviewing our catalog, it seems that we don't have any antibodies that would be likely to cross-react with the guinea pig Interferon gamma sequence you provided.  Of the antibodies we have, the closest match I could fin...

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Thank you for contacting us.  I was unable to locate an amino acid sequence for guinea pig IFN-gamma.  If you could please provide one, I would be happy to check our antibodies to see if we have one that would be likely to react.

Thank you for your enquiry. The originator of the antibody confirmed that gels for Western blotting with this antibody should be run using non-reducing conditions, as we suspected. I have added this information to the datasheet for future reference. ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"