• Product nameAnti-Interferon gamma antibody [EP1109Y]
    See all Interferon gamma primary antibodies
  • Description
    Rabbit monoclonal [EP1109Y] to Interferon gamma
  • SpecificityThis antibody reacts with Interferon gamma.
  • Tested applicationsSuitable for: WBmore details
    Unsuitable for: ICC/IF or IHC-P
  • Species reactivity
    Reacts with: Human
  • Immunogen

    A synthetic peptide corresponding to residues of human Interferon gamma.

  • Positive control
    • Human Interferon gamma recombinant protein
  • General notes

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated ‘PUR’ on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    A trial size is available to purchase for this antibody.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.



Our Abpromise guarantee covers the use of ab52746 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/2000. Detects a band of approximately 17 kDa (predicted molecular weight: 19 kDa).
  • Application notesIs unsuitable for ICC/IF or IHC-P.
  • Target

    • FunctionProduced by lymphocytes activated by specific antigens or mitogens. IFN-gamma, in addition to having antiviral activity, has important immunoregulatory functions. It is a potent activator of macrophages, it has antiproliferative effects on transformed cells and it can potentiate the antiviral and antitumor effects of the type I interferons.
    • Tissue specificityReleased primarily from activated T lymphocytes.
    • Involvement in diseaseIn Caucasians, genetic variation in IFNG is associated with the risk of aplastic anemia (AA) [MIM:609135]. AA is a rare disease in which the reduction of the circulating blood cells results from damage to the stem cell pool in bone marrow. In most patients, the stem cell lesion is caused by an autoimmune attack. T-lymphocytes, activated by an endogenous or exogenous, and most often unknown antigenic stimulus, secrete cytokines, including IFN-gamma, which would in turn be able to suppress hematopoiesis.
    • Sequence similaritiesBelongs to the type II (or gamma) interferon family.
    • Post-translational
      Proteolytic processing produces C-terminal heterogeneity, with proteins ending alternatively at Gly-150, Met-157 or Gly-161.
    • Cellular localizationSecreted.
    • Information by UniProt
    • Database links
    • Alternative names
      • IFG antibody
      • IFI antibody
      • IFN gamma antibody
      • IFN, immune antibody
      • IFN-gamma antibody
      • IFNG antibody
      • IFNG_HUMAN antibody
      • Immune interferon antibody
      • Interferon gamma antibody
      see all

    Anti-Interferon gamma antibody [EP1109Y] images

    • Anti-Interferon gamma antibody [EP1109Y] (ab52746) at 1/1000 dilution + Human Interferon gamma recombinant protein at 0.015 µg

      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size : 19 kDa

      Blocking buffer: 5% NFDM/TBST

      Diluting buffer: 5% NFDM/TBST

    • Anti-Interferon gamma antibody [EP1109Y] (ab52746) at 1/2000 dilution + recombinant protein at 0.01 µg

      Goat anti-rabbit HRP labeled at 1/2000 dilution

      Predicted band size : 19 kDa
      Observed band size : 17 kDa (why is the actual band size different from the predicted?)

    References for Anti-Interferon gamma antibody [EP1109Y] (ab52746)

    This product has been referenced in:
    • Lian X  et al. Vibrio parahaemolyticus elevates interferon alpha production in intestinal-like epithelial Caco-2 cells. Can J Microbiol 53:1084-90 (2007). Read more (PubMed: 18026229) »

    See 1 Publication for this product

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