Anti-Interferon gamma antibody [GIF-7] (ab9808)

Overview

  • Product nameAnti-Interferon gamma antibody [GIF-7]
    See all Interferon gamma primary antibodies
  • Description
    Mouse monoclonal [GIF-7] to Interferon gamma
  • SpecificityRecognizes human IFN-g. Does not react with human IFN-a.
  • Tested applicationsSuitable for: ELISA, Indirect ELISA, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant full length protein (Human).

Properties

Applications

Our Abpromise guarantee covers the use of ab9808 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA
Indirect ELISA
WB
  • Application notesIndirect ELISA: use at 1/1000 to 1/5000
    Western Blot: use at 1/500 to 1/2000

  • Target

    • FunctionProduced by lymphocytes activated by specific antigens or mitogens. IFN-gamma, in addition to having antiviral activity, has important immunoregulatory functions. It is a potent activator of macrophages, it has antiproliferative effects on transformed cells and it can potentiate the antiviral and antitumor effects of the type I interferons.
    • Tissue specificityReleased primarily from activated T lymphocytes.
    • Involvement in diseaseIn Caucasians, genetic variation in IFNG is associated with the risk of aplastic anemia (AA) [MIM:609135]. AA is a rare disease in which the reduction of the circulating blood cells results from damage to the stem cell pool in bone marrow. In most patients, the stem cell lesion is caused by an autoimmune attack. T-lymphocytes, activated by an endogenous or exogenous, and most often unknown antigenic stimulus, secrete cytokines, including IFN-gamma, which would in turn be able to suppress hematopoiesis.
    • Sequence similaritiesBelongs to the type II (or gamma) interferon family.
    • Post-translational
      modifications
      Proteolytic processing produces C-terminal heterogeneity, with proteins ending alternatively at Gly-150, Met-157 or Gly-161.
    • Cellular localizationSecreted.
    • Information by UniProt
    • Database links
    • Alternative names
      • IFG antibody
      • IFI antibody
      • IFN gamma antibody
      • IFN, immune antibody
      • IFN-gamma antibody
      • IFNG antibody
      • IFNG_HUMAN antibody
      • Immune interferon antibody
      • Interferon gamma antibody
      see all

    Anti-Interferon gamma antibody [GIF-7] images

    • Western blot against human interferon gamma using ab9808 at 1 µg/ml.

    References for Anti-Interferon gamma antibody [GIF-7] (ab9808)

    ab9808 has not yet been referenced specifically in any publications.

    Product Wall

    Thank you for your enquiry. We do not have a Western blot image with molecular weight markers for this antibody. Interferon gamma precursor is 19 kD and can be expected to run on an SDS-PAGE gel at 17 - 20 kD. The Western blot band may appear broad...

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    Thank you for your enquiry. Regarding the Western blot for ab9808: Protein (interferon-gamma) loading was 200 ng per lane. Ab9808 was used at a concentration of 1 microgram/ml. Secondary antibody: anti-mouse IgG, HRP-labeled, 1:1000 Substrate: ...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"