Anti-Interferon gamma antibody [RMMG-1] (ab24979)

Overview

Properties

Applications

Our Abpromise guarantee covers the use of ab24979 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Neutralising Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Predicted molecular weight: 19 kDa.
IP Use at an assay dependent concentration.
ELISA Use at an assay dependent concentration.
ELISpot Use at an assay dependent concentration.

Target

  • FunctionProduced by lymphocytes activated by specific antigens or mitogens. IFN-gamma, in addition to having antiviral activity, has important immunoregulatory functions. It is a potent activator of macrophages, it has antiproliferative effects on transformed cells and it can potentiate the antiviral and antitumor effects of the type I interferons.
  • Tissue specificityReleased primarily from activated T lymphocytes.
  • Involvement in diseaseIn Caucasians, genetic variation in IFNG is associated with the risk of aplastic anemia (AA) [MIM:609135]. AA is a rare disease in which the reduction of the circulating blood cells results from damage to the stem cell pool in bone marrow. In most patients, the stem cell lesion is caused by an autoimmune attack. T-lymphocytes, activated by an endogenous or exogenous, and most often unknown antigenic stimulus, secrete cytokines, including IFN-gamma, which would in turn be able to suppress hematopoiesis.
  • Sequence similaritiesBelongs to the type II (or gamma) interferon family.
  • Post-translational
    modifications
    Proteolytic processing produces C-terminal heterogeneity, with proteins ending alternatively at Gly-150, Met-157 or Gly-161.
  • Cellular localizationSecreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • IFG antibody
    • IFI antibody
    • IFN gamma antibody
    • IFN, immune antibody
    • IFN-gamma antibody
    • IFNG antibody
    • IFNG_HUMAN antibody
    • Immune interferon antibody
    • Interferon gamma antibody
    see all

References for Anti-Interferon gamma antibody [RMMG-1] (ab24979)

This product has been referenced in:
  • Yamamoto-Taguchi N  et al. HTLV-1 bZIP factor induces inflammation through labile Foxp3 expression. PLoS Pathog 9:e1003630 (2013). IHC-Fr ; Mouse . Read more (PubMed: 24068936) »
  • Ma Y  et al. Toll-like receptor (TLR) 2 and TLR4 differentially regulate doxorubicin induced cardiomyopathy in mice. PLoS One 7:e40763 (2012). IHC-P ; Mouse . Read more (PubMed: 22808256) »

See all 7 Publications for this product

Product Wall

Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (brain (hypothlamus))
Permeabilization Yes - PBST
Specification brain (hypothlamus)
Fixative Paraformaldehyde
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Submitted Sep 11 2015

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2.5% · Temperature: 25°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Trilogy
Sample Mouse Tissue sections (duodenum)
Specification duodenum
Permeabilization No
Fixative Formaldehyde
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Submitted Jan 10 2014



La méthode de purification de l'anticorps ab24979est une combinaison de chromatographie échangeuse d'ions,interactions hydrophobiques et chromatographie d'exclusion.

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (Lung)
Specification Lung
Fixative Formaldehyde
Antigen retrieval step None
Permeabilization Yes - 0.2% Triton X-100 in PBS
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C
Username

Ms. Ya-Jing Hsu

Verified customer

Submitted Jan 24 2012

DISCOUNT CODE: xxxxxx Expiration date: 23/02/2012 I am very pleased to hear you would like to accept our offer and test ab24979 in IHC-P. This code will give you 1 free primary antibody before the expiration date. To redeem this offer, please sub...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Tissue lysate - whole (lung homogenate)
Loading amount 30 µg
Specification lung homogenate
Gel Running Conditions Non-reduced Denaturing (4-20%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C
Username

Abcam user community

Verified customer

Submitted Mar 06 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"