Anti-Interferon gamma antibody [RMMG-1] (ab24979)

Overview

  • Product name
    Anti-Interferon gamma antibody [RMMG-1]
    See all Interferon gamma primary antibodies
  • Description
    Rat monoclonal [RMMG-1] to Interferon gamma
  • Specificity
    ab24979 recognises Interferon gamma.
  • Tested applications
    Suitable for: Neutralising, WB, IP, ELISA, ELISpotmore details
  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    Recombinant full length protein (Mouse): interferon gamma

  • Positive control
  • General notes

    We have had both positive and negative feedback from customers using this antibody in IHC-P. In light of this data, we cannot guarantee this antibody for IHC-P at this time.

    Abcam is committed to meeting high standards of ethical manufacturing and as such, we will be discontinuing this product, which has been generated by the ascites method, within the next year. We are sorry for any inconvenience this may cause. If you would like help finding an alternative product, please do not hesitate to contact our scientific support team.

Properties

Applications

Our Abpromise guarantee covers the use of ab24979 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Neutralising Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Predicted molecular weight: 19 kDa.
IP Use at an assay dependent concentration.
ELISA Use at an assay dependent concentration.
ELISpot Use at an assay dependent concentration.

Target

  • Function
    Produced by lymphocytes activated by specific antigens or mitogens. IFN-gamma, in addition to having antiviral activity, has important immunoregulatory functions. It is a potent activator of macrophages, it has antiproliferative effects on transformed cells and it can potentiate the antiviral and antitumor effects of the type I interferons.
  • Tissue specificity
    Released primarily from activated T lymphocytes.
  • Involvement in disease
    In Caucasians, genetic variation in IFNG is associated with the risk of aplastic anemia (AA) [MIM:609135]. AA is a rare disease in which the reduction of the circulating blood cells results from damage to the stem cell pool in bone marrow. In most patients, the stem cell lesion is caused by an autoimmune attack. T-lymphocytes, activated by an endogenous or exogenous, and most often unknown antigenic stimulus, secrete cytokines, including IFN-gamma, which would in turn be able to suppress hematopoiesis.
  • Sequence similarities
    Belongs to the type II (or gamma) interferon family.
  • Post-translational
    modifications
    Proteolytic processing produces C-terminal heterogeneity, with proteins ending alternatively at Gly-150, Met-157 or Gly-161.
  • Cellular localization
    Secreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • IFG antibody
    • IFI antibody
    • IFN gamma antibody
    • IFN, immune antibody
    • IFN-gamma antibody
    • IFNG antibody
    • IFNG_HUMAN antibody
    • Immune interferon antibody
    • Interferon gamma antibody
    see all

References

This product has been referenced in:
  • Lv YG  et al. Fluorosis increases the risk of postmenopausal osteoporosis by stimulating interferon ?. Biochem Biophys Res Commun 479:372-379 (2016). WB ; Mouse . Read more (PubMed: 27644876) »
  • Khalaj K  et al. RNA binding protein, tristetraprolin in a murine model of recurrent pregnancy loss. Oncotarget 7:72486-72502 (2016). WB ; Mouse . Read more (PubMed: 27732963) »

See all 10 Publications for this product

Customer reviews and Q&As

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (pancreatic cancer)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Target Retrieval Solution ph 6.0
Permeabilization
No
Specification
pancreatic cancer
Fixative
Formaldehyde
Username

Pan Pan

Verified customer

Submitted Apr 17 2017

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (brain (hypothlamus))
Permeabilization
Yes - PBST
Specification
brain (hypothlamus)
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Sep 11 2015

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (duodenum)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Trilogy
Permeabilization
No
Specification
duodenum
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2.5% · Temperature: 25°C
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

Submitted Jan 10 2014



La méthode de purification de l'anticorps ab24979est une combinaison de chromatographie échangeuse d'ions,interactions hydrophobiques et chromatographie d'exclusion.

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Lung)
Antigen retrieval step
None
Permeabilization
Yes - 0.2% Triton X-100 in PBS
Specification
Lung
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C
Fixative
Formaldehyde
Username

Ms. Ya-Jing Hsu

Verified customer

Submitted Jan 24 2012

DISCOUNT CODE: xxxxxx Expiration date: 23/02/2012 I am very pleased to hear you would like to accept our offer and test ab24979 in IHC-P. This code will give you 1 free primary antibody before the expiration date. To redeem this offer, please sub...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Tissue lysate - whole (lung homogenate)
Loading amount
30 µg
Specification
lung homogenate
Gel Running Conditions
Non-reduced Denaturing (4-20%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C
Username

Abcam user community

Verified customer

Submitted Mar 06 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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