The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent concentration.
Use at an assay dependent concentration. ab18404-Rat monoclonal IgG1, is suitable for use as an isotype control with this antibody.
Use at an assay dependent concentration. Ab87303 can be used as a capture antibody in a sandwich ELISA for quantifying mouse IFN-? protein levels. Biotinylated form of the antibody can be paired with a suitable purified capture antibody, with purified recombinant human IFN-? as the standard.
FunctionProduced by lymphocytes activated by specific antigens or mitogens. IFN-gamma, in addition to having antiviral activity, has important immunoregulatory functions. It is a potent activator of macrophages, it has antiproliferative effects on transformed cells and it can potentiate the antiviral and antitumor effects of the type I interferons.
Tissue specificityReleased primarily from activated T lymphocytes.
Involvement in diseaseIn Caucasians, genetic variation in IFNG is associated with the risk of aplastic anemia (AA) [MIM:609135]. AA is a rare disease in which the reduction of the circulating blood cells results from damage to the stem cell pool in bone marrow. In most patients, the stem cell lesion is caused by an autoimmune attack. T-lymphocytes, activated by an endogenous or exogenous, and most often unknown antigenic stimulus, secrete cytokines, including IFN-gamma, which would in turn be able to suppress hematopoiesis.
Sequence similaritiesBelongs to the type II (or gamma) interferon family.
Post-translational modificationsProteolytic processing produces C-terminal heterogeneity, with proteins ending alternatively at Gly-150, Met-157 or Gly-161.
References for Anti-Interferon gamma antibody [XMG1.2] (FITC) (ab87030)
This product has been referenced in:
Vikingsson A et al. Altered kinetics of CD4+ T cell proliferation and interferon-gamma production in the absence of CD8+ T lymphocytes in virus-infected beta2-microglobulin-deficient mice. Cell Immunol173:261-8 (1996).
Read more (PubMed: 8912885) »
Hsieh B et al. In vivo cytokine production in murine listeriosis. Evidence for immunoregulation by gamma delta+ T cells. J Immunol156:232-7 (1996).
Read more (PubMed: 8598467) »