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Interferon gamma Human ELISA Kit (ab46045)

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Overview

Product name

Interferon gamma Human ELISA Kit
See all Interferon gamma products (13) ...

Specificity

Recognizes both natural and recombinant human IFN-gRange: 12.5 pg/ml - 400 pg/mlSensitivity <5 pg/ml

Precision

Intra-assay
Sample n Mean SD CV%
Overall 4.9%
Inter-assay
Sample n Mean SD CV%
Sample 1 18 264pg/ml 16 6%
Sample 2 18 138pg/ml 11 8%
Sample 3 18 56pg/ml 8 14%
Sample 4 18 75pg/ml 11 14%
Sample 5 18 180pg/ml 16 9%

Tests

2 x 96 well plate

Sample type

Cell culture supernatant, Serum, Plasma

Assay type

Sandwich

Sensitivity

< 5 pg/ml

Range

12.5 pg/ml - 400 pg/ml

Recovery

107%

Assay time

2h 40m

Cross reactivity

Reacts with

Human

Product overview

The Human Interferon gamma ELISA is to be used for the in-vitro quantitative determination of interferon gamma (Interferon gamma) in human serum, plasma, buffered solutions or cell culture medium. The assay will recognize both natural and recombinant human Interferon gamma.

Principal:

A monoclonal antibody specific for Interferon gamma has been coated onto the wells of the microtiter strips provided. During the first incubation, Interferon gamma present in the sample or standard and a monoclonal anti-Interferon gamma antibody conjugated to biotin are simultaneaously incubated. Following incubation unbound biotinylated anti-Interferon gamma is removed during a wash step. Streptavidin-HRP is added and binds to the biotinylated anti-Interferon gamma. After incubation and a wash step a substrate solution reactive with HRP is added to the wells. A coloured product is formed in proportion to the amount of Interferon gamma present in the sample. The reaction is terminated by addition of acid and absorbance is measured at 450 nm.

Notes

Safety Notes:

  • For research use only.
  • The human blood components included in this kit have been tested and found non reactive for HBsAg and anti-HIV. Nevertheless, no known method can offer complete assurance that human blood derivatives will not transmit hepatitis, AIDS or other infections. Therefore, handling of reagents, serum or plasma specimens should be in accordance with local safety procedures , e.g. CDC/NIH Health manual : " Biosafety in Microbiological and Biomedical Laboratories" 1984.
  • Avoid any skin contact with H2SO4 and TMB. In case of contact, wash thoroughly with water.
  • Do not eat, drink, smoke or apply cosmetics where kit reagents are used.
  • Do not pipette by mouth.
  • The stop solution in this kit contains 9.8% sulphuric acid.

Tested applications

ELISAmore details

Properties

Storage instructions

Store at 4°C. The kit is shipped at room temperature

Components 2 x 96 tests
12 strips8 units
Biotinylated Antibody Diluent1 x 13ml
Biotinylated anti-IFN gamma2 x 0.4ml
Control4 vials
HRP Diluent1 x 23ml
Standard4 vials
Standard Diluent : human serum2 x 7ml
Standard Diluent Buffer.x10 Concentrated1 x 25ml
Stop Reagent: H2SO42 x 11ml
Streptavidin-HRP4 x 5µl
Substrate Solution; chromogen TMB1 x 24ml
Washing Buffer.x200 Concentrated2 x 10ml

Research areas

Kits/ Lysates/ Other >> Kits >> ELISA and ELIPAIR Kits >> ELISA Kits >> Cytokines and cytokine receptors ELISA kits
Cancer >> Tumor immunology >> Cytokines >> Interferons
Stem Cells >> Signaling Pathways >> TGF beta >> Interferon gamma
Immunology >> Innate Immunity >> Cytokines >> Interferons

  • ELISA - Human Interferon gamma ELISA Kit (ab46045)ELISA - Human Interferon gamma ELISA Kit (ab46045) image (enlarge)

Relevance

Mammalian Interferon gamma is mainly produced by T lymphocytes and NK cells. It is a pleiotropic cytokine involved in the regulation of nearly all phases of immune and inflammatory responses,including the activation, growth and differentiation of T cell, B cells, macrophages, NK cells and other cell types such as endothelial cells and fibroblasts. It has weak antiviral and antiproliferative activity, and poteniates the antiviral and anti tumor effects of IFN alpha / beta (type I interferon). It is upregulated by IL2, FGF basic, EGF and downregulated by vitamin D3 or DMN. Labile at pH 2.

Alternative names

  • IF 1
  • IFG
  • IFI
  • IFN gamma
  • IFN Immune
  • IFNG
  • Immune interferon
  • Interferon gamma
  • Interferon Gamma Precursor
  • Macrophage Activating Factor
  • MAF
  • T Cell Interferon
  • Type II Interferon
see all

Database links

Applications

Show applications key

Our Abpromise guarantee covers the use of ab46045 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Interferon gamma Human ELISA Kit images:

  ELISA - Human Interferon gamma ELISA Kit (ab46045)

ELISA - Human Interferon gamma ELISA Kit (ab46045)

Typical standard curve for Interferon gamma ELISA ranging from 12.5 to 400 pg/ml.

Protocols

  • Human Interferon gamma ELISA Kit

    • References for Interferon gamma Human ELISA Kit (ab46045)

    ab46045 has not yet been referenced specifically in any publications.

    Publishing research using ab46045? Please let us know so that we can cite the reference in this datasheet

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"