Interferon gamma Human ELISA Set (without plates) (ab48490)

Overview

  • Product nameInterferon gamma Human ELISA Set (without plates)
    See all Interferon gamma kits
  • Tests
    5 x 96 well plate
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay typeSandwich (quantitative)
  • Sensitivity
    < 5 pg/ml
  • Range
    400 pg/ml - 12.5 pg/ml
  • Assay durationMultiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s IFNγ Human in vitro ELISA Set is designed for the quantitative measurement of IFNγ in cell culture supernatants, buffered solutions, serum, plasma and other body fluids.

     

    The Interferon gamma ELISA Set includes both the capture and the detection antibodies as well as Horse-Radish peroxidase conjugated Streptavidin (HRP-Strep) and TMB which are the reagents used in the colour development step.

     

    This ELISA Set is intended to measure human Interferon gamma in cell -culture supernatants, as well as in serum and plasma samples. The enclosed procedures provide general guidelines for ELISA preparation. This procedure may need some optimization depending on the plastic, matrix, standard range.

      

    Sensitivity: < 5 pg / ml

  • Tested applicationsSandwich ELISAmore details
  • PlatformReagents

Properties

  • RelevanceIFN-gamma is produced by lymphocytes activated by specific antigens or mitogens. IFN-gamma, in addition to having antiviral activity, has important immunoregulatory functions. It is a potent activator of macrophages, it has antiproliferative effects on transformed cells and it can potentiate the antiviral and antitumor effects of the type I interferons.
  • Cellular localizationSecreted
  • Alternative names
    • IFG
    • IFI
    • IFN gamma
    • IFNG
    • Interferon gamma
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab48490 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA
  • Application notesELISA: Use at an assay dependent dilution.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Interferon gamma Human ELISA Set (without plates) images

    • IFNgamma Standard curve ranging from 12.5 to 400 pg/mL

    References for Interferon gamma Human ELISA Set (without plates) (ab48490)

    ab48490 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"