Anti-Interferon Receptor alpha antibody [EPR6244] (ab124764)

Overview

  • Product nameAnti-Interferon Receptor alpha antibody [EPR6244]
    See all Interferon Receptor alpha primary antibodies
  • Description
    Rabbit monoclonal [EPR6244] to Interferon Receptor alpha
  • SpecificityIn mouse and rat tissue lysates this product detects a band in the region of 100 kDa, however we believe this band is non-specific and is not interferon receptor alpha as the immunogen for this antibody shares only 55% homology with the mouse and rat protein. In addition this band also migrates at a lower molecular weight than that detected in human samples.
  • Tested applicationsSuitable for: WBmore details
    Unsuitable for: ICC/IF or IHC-P
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human Interferon Receptor alpha aa 100-200.

  • Positive control
    • HeLa, HeLa treated with IFN-alpha, K562 and U937 cell lysates.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated ‘PUR’ on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab124764 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 90-130 kDa (predicted molecular weight: 64 kDa).
  • Application notesIs unsuitable for ICC/IF or IHC-P.
  • Target

    • RelevanceInterferon Receptor alpha (IFN-a R1) is a class II cytokine receptor which belongs to type I human interferons (IFNs) family. IFNs plays a role in antiviral, antiproliferative, immunomodulatory, antitumor, and antiparasitic activities by inducing transcription of IFN-stimuated genes (ISGs) through activation of the Jak-STAT pathway. Specifically, Interferon Receptor alpha is a 135-kda signal transducing subunit of the IFN alpha/beta receptor complex (IFN-a R). Interferon Receptor alpha is phospholyated through PTK- and PKC-mediated phosphorylation, which initiates docking of STAT proteins to IFNs. This leads to activation of STAT proteins by PTK-mediated phosphorylation. The mechanism behind the amplification of Interferon Receptor alpha complex mediated transmembrane signaling has been linked to tyrosine phosphorylation on Interferon Receptor alpha. IFNAR1 possesses a large N-glycosylated ectodomain.
    • Cellular localizationMembrane; single-pass type I membrane protein.
    • Database links
    • Alternative names
      • AVP antibody
      • IFN alpha R1 antibody
      • IFN alpha REC antibody
      • IFNAR antibody
      • IFNAR1 antibody
      • IFNBR antibody
      • IFRC antibody
      • interferon (alpha beta and omega) receptor 1 antibody
      • Interferon alpha receptor 1 antibody
      • Interferon alpha/beta receptor alpha chain antibody
      see all

    Anti-Interferon Receptor alpha antibody [EPR6244] images

    • All lanes : Anti-Interferon Receptor alpha antibody [EPR6244] (ab124764) at 1/1000 dilution (purified)

      Lane 1 : Mouse brain lysate
      Lane 2 : Rat brain lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      Rabbit monoclonal [EPR6244] to Interferon Receptor alpha (ab124764) at 1/1000 dilution

      Predicted band size : 64 kDa
      Observed band size : 90 kDa (why is the actual band size different from the predicted?)

      Blocking buffer: 5% NFDM/TBST
      Dilution buffer: 5% NFDM/TBST

    • All lanes : Anti-Interferon Receptor alpha antibody [EPR6244] (ab124764) at 1/1000 dilution (purified)

      Lane 1 : HEK293 whole cell lysate
      Lane 2 : HeLa whole cell lysate
      Lane 3 : LNCaP whole cell lysate
      Lane 4 : SH-SY5Y whole cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size : 64 kDa
      Observed band size : 90-130 kDa (why is the actual band size different from the predicted?)

      Blocking buffer: 5% NFDM/TBST
      Dilution buffer: 5% NFDM/TBST

    • All lanes : Anti-Interferon Receptor alpha antibody [EPR6244] (ab124764) at 1/1000 dilution (unpurified)

      Lane 1 : Mouse brain whole cell lysate
      Lane 2 : Mouse heart whole cell lysate
      Lane 3 : Mouse kidney whole cell lysate
      Lane 4 : Mouse spleen whole cell lysate
      Lane 5 : Rat brain whole cell lysate
      Lane 6 : Rat heart whole cell lysate
      Lane 7 : Rat kidney whole cell lysate
      Lane 8 : Rat spleen whole cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat anti-rabbit IgG (H+L), peroxidase conjugated. at 1/2000 dilution

      Predicted band size : 64 kDa


      Exposure time : 3 minutes

      Blocking buffer: 5% NFDM/TBST.

      In mouse and rat tissue lysates this product detects a band in the region of 100 kDa, however we believe this band is non-specific and is not interferon receptor alpha as the immunogen for this antibody shares only 55% homology with the mouse and rat protein. In addition this band also migrates at a lower molecular weight than that detected in human samples.

    • All lanes : Anti-Interferon Receptor alpha antibody [EPR6244] (ab124764) at 1/1000 dilution (Unpurified)

      Lane 1 : HeLa cell lysate
      Lane 2 : HeLa cell lysate treated with IFN-alpha
      Lane 3 : K562 cell lysate
      Lane 4 : U937 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat anti-Rabbit HRP at 1/200 dilution

      Predicted band size : 64 kDa
    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References for Anti-Interferon Receptor alpha antibody [EPR6244] (ab124764)

    ab124764 has not yet been referenced specifically in any publications.

    Product Wall

    Application Immunocytochemistry/ Immunofluorescence
    Sample Human Cell (human retinal epithelioïd cells)
    Specification human retinal epithelioïd cells
    Fixative Paraformaldehyde
    Permeabilization No
    Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 0.02% · Temperature: RT°C
    Username

    Abcam user community

    Verified customer

    Submitted May 03 2013

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Human Cell lysate - whole cell (hTERT-RPE1)
    Loading amount 150000 cells
    Specification hTERT-RPE1
    Gel Running Conditions Reduced Denaturing (8 % acrylamid)
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
    Username

    Abcam user community

    Verified customer

    Submitted Apr 16 2013

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"