Anti-Interferon regulatory factor 9 antibody (ab126940)

Overview

  • Product name
    Anti-Interferon regulatory factor 9 antibody
    See all Interferon regulatory factor 9 primary antibodies
  • Description
    Rabbit polyclonal to Interferon regulatory factor 9
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 171-354 of Human Interferon regulatory factor 9 (Q00978).

  • Positive control
    • A549, HeLa, HepG2 and HCT116 whole cell lysates; HeLa cells; Human Breast carcinoma tissue.
  • General notes
    Keep as concentrated solution.

Properties

Applications

Our Abpromise guarantee covers the use of ab126940 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 44 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/100 - 1/1000.

Target

  • Function
    Transcription regulatory factor that mediates signaling by type I IFNs (IFN-alpha and IFN-beta). Following type I IFN binding to cell surface receptors, Jak kinases (TYK2 and JAK1) are activated, leading to tyrosine phosphorylation of STAT1 and STAT2. The phosphorylated STATs dimerize, associate with IRF9/ISGF3G to form a complex termed ISGF3 transcription factor, that enters the nucleus. ISGF3 binds to the IFN stimulated response element (ISRE) to activate the transcription of interferon stimulated genes, which drive the cell in an antiviral state.
  • Sequence similarities
    Belongs to the IRF family.
    Contains 1 IRF tryptophan pentad repeat DNA-binding domain.
  • Cellular localization
    Cytoplasm. Nucleus. Translocated into the nucleus upon activation by IFN-alpha/beta.
  • Information by UniProt
  • Database links
  • Alternative names
    • IFN alpha responsive transcription factor subunit antibody
    • IFN-alpha-responsive transcription factor subunit antibody
    • Interferon regulatory factor 9 antibody
    • interferon stimulated transcription factor 3 antibody
    • Interferon-stimulated gene factor 3 gamma antibody
    • interferon-stimulated transcription factor 3, gamma 48kDa antibody
    • IRF 9 antibody
    • IRF-9 antibody
    • Irf9 antibody
    • IRF9_HUMAN antibody
    • ISGF 3 gamma antibody
    • ISGF-3 gamma antibody
    • ISGF3 antibody
    • ISGF3 p48 subunit antibody
    • ISGF3G antibody
    • OTTHUMP00000164692 antibody
    • OTTHUMP00000164693 antibody
    • p48 antibody
    • Transcriptional regulator ISGF3 subunit gamma antibody
    see all

Images

  • All lanes : Anti-Interferon regulatory factor 9 antibody (ab126940) at 1/5000 dilution

    Lane 1 : A549 whole cell lysate
    Lane 2 : HeLa whole cell lysate

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 44 kDa
  • ab126940, at 1/250, staining Interferon regulatory factor 9 in paraffin-embedded Human Breast carcinoma tissue by Immunohistochemistry.
  • ab126940, at 1/500, staining Interferon regulatory factor 9 in paraformaldehyde-fixed HeLa cells by confocal immunofluorescence. Alpha-tubulin filaments are stained red.

References

ab126940 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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