Recombinant
RabMAb

Anti-Interferon regulatory factor 9 antibody [EPR3939] (ab109309)

Overview

Properties

Applications

Our Abpromise guarantee covers the use of ab109309 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC 1/100 - 1/250.
WB 1/10000 - 1/50000. Detects a band of approximately 48 kDa (predicted molecular weight: 44 kDa).
Flow Cyt 1/10 - 1/100. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
  • Application notes
    Is unsuitable for IHC-P or IP.
  • Target

    • Function
      Transcription regulatory factor that mediates signaling by type I IFNs (IFN-alpha and IFN-beta). Following type I IFN binding to cell surface receptors, Jak kinases (TYK2 and JAK1) are activated, leading to tyrosine phosphorylation of STAT1 and STAT2. The phosphorylated STATs dimerize, associate with IRF9/ISGF3G to form a complex termed ISGF3 transcription factor, that enters the nucleus. ISGF3 binds to the IFN stimulated response element (ISRE) to activate the transcription of interferon stimulated genes, which drive the cell in an antiviral state.
    • Sequence similarities
      Belongs to the IRF family.
      Contains 1 IRF tryptophan pentad repeat DNA-binding domain.
    • Cellular localization
      Cytoplasm. Nucleus. Translocated into the nucleus upon activation by IFN-alpha/beta.
    • Information by UniProt
    • Database links
    • Alternative names
      • IFN alpha responsive transcription factor subunit antibody
      • IFN-alpha-responsive transcription factor subunit antibody
      • Interferon regulatory factor 9 antibody
      • interferon stimulated transcription factor 3 antibody
      • Interferon-stimulated gene factor 3 gamma antibody
      • interferon-stimulated transcription factor 3, gamma 48kDa antibody
      • IRF 9 antibody
      • IRF-9 antibody
      • Irf9 antibody
      • IRF9_HUMAN antibody
      • ISGF 3 gamma antibody
      • ISGF-3 gamma antibody
      • ISGF3 antibody
      • ISGF3 p48 subunit antibody
      • ISGF3G antibody
      • OTTHUMP00000164692 antibody
      • OTTHUMP00000164693 antibody
      • p48 antibody
      • Transcriptional regulator ISGF3 subunit gamma antibody
      see all

    Images

    • All lanes : Anti-Interferon regulatory factor 9 antibody [EPR3939] (ab109309) at 1/1000 dilution

      Lane 1 : K562 treated with IFN-alpha cell lysates
      Lane 2 : K562 cell lysates
      Lane 3 : HeLa treated with IFN-alpha cell lysates
      Lane 4 : HeLa cell lysates
      Lane 5 : Jurkat cell lysates

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 44 kDa
      Observed band size : 48 kDa (why is the actual band size different from the predicted?)
    • Flow cytometric analysis of permeabilized Jurkat cells using ab109309 at 1/10 dilution (red)and Rabbit IgG negative control (green).

    References

    ab109309 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Thank you for your telephone call yesterday afternoon.I am sorry to hear you have had difficulty obtaining satisfactory results from this antibody.

    I would like to reassure you that this antibody is tested and covered by our 6 month guarante...

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

    Sign up