• Product nameAnti-Intersectin 1 antibody
  • Description
    Rabbit polyclonal to Intersectin 1
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Recombinant fragment, corresponding to amino acids 646-840 of Human Intersectin 1 (BC116186).

  • Positive control
    • Human fetal brain lysate



Our Abpromise guarantee covers the use of ab118262 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 195 kDa.


  • FunctionAdapter protein that may provide indirect link between the endocytic membrane traffic and the actin assembly machinery. May regulate the formation of clathrin-coated vesicles. Isoform 1 could be involved in brain-specific synaptic vesicle recycling. Inhibits ARHGAP31 activity toward RAC1.
  • Tissue specificityIsoform 2 is ubiquitous in adult and fetal tissues with high expression in skeletal muscle, heart, spleen, ovary, testis and all fetal tissues tested and low expression in thymus, blood, lung, liver and pancreas. Isoform 1 is expressed almost exclusively in the brain, in all brain regions. Not expressed in the spinal cord.
  • Sequence similaritiesContains 1 C2 domain.
    Contains 1 DH (DBL-homology) domain.
    Contains 2 EF-hand domains.
    Contains 2 EH domains.
    Contains 1 PH domain.
    Contains 5 SH3 domains.
  • DomainSH3-3, SH3-4 and SH3-5, but not SH3-1 and SH3-2 domains, bind to dynamin (By similarity). SH3-1 and SH3-4 bind to ARHGAP31.
    The KLERQ domain binds to SNAP-25 and SNAP-23.
  • Cellular localizationEndomembrane system. Cell junction > synapse > synaptosome. Cell projection > lamellipodium.
  • Information by UniProt
  • Database links
  • Alternative names
    • Human intersectin SH3 domain containing protein SH3P17 antibody
    • Intersectin 1 SH3 domain protein antibody
    • intersectin 1 short form variant 3 antibody
    • intersectin 1 short form variant, 11 antibody
    • Intersectin short form antibody
    • intersectin short variant 12 antibody
    • Intersectin-1 antibody
    • Intersectin1 antibody
    • ITSN 1 antibody
    • ITSN antibody
    • itsn1 antibody
    • ITSN1_HUMAN antibody
    • MGC134948 antibody
    • MGC134949 antibody
    • OTTHUMP00000067855 antibody
    • OTTHUMP00000067856 antibody
    • OTTHUMP00000067857 antibody
    • OTTHUMP00000194810 antibody
    • OTTHUMP00000194811 antibody
    • OTTHUMP00000194812 antibody
    • OTTHUMP00000206688 antibody
    • SH3 domain containing protein 1A antibody
    • SH3 domain protein 1A antibody
    • SH3 domain-containing protein 1A antibody
    • SH3D1A antibody
    • SH3P17 antibody
    • Src homology 3 domain containing protein antibody
    see all

Anti-Intersectin 1 antibody images

  • Anti-Intersectin 1 antibody (ab118262) at 1/500 dilution + Human fetal brain lysate

    Predicted band size : 195 kDa
    Additional bands at : 129 kDa (possible isoform),138 kDa (possible isoform).

References for Anti-Intersectin 1 antibody (ab118262)

This product has been referenced in:
  • Humphries AC  et al. Cdc42 and the Rho GEF intersectin-1 collaborate with Nck to promote N-WASP-dependent actin polymerisation. J Cell Sci 127:673-85 (2014). Read more (PubMed: 24284073) »

See 1 Publication for this product

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - whole cell (Dorsal root ganglion)
Gel Running Conditions Reduced Denaturing (12.5)
Loading amount 25 µg
Treatment Mutants
Specification Dorsal root ganglion
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C

Anu Shanu

Verified customer

Submitted Mar 24 2017