Recombinant Anti-I-FABP antibody [EPR7136] (ab128860)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR7136] to I-FABP
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-I-FABP antibody [EPR7136]
See all I-FABP primary antibodies -
Description
Rabbit monoclonal [EPR7136] to I-FABP -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide within Human I-FABP aa 1-100. The exact sequence is proprietary.
Database link: P12104 -
Positive control
- WB: Human, Rat and Mouse small intestine lysates.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Dissociation constant (KD)
KD = 2.90 x 10 -11 M Learn more about KD -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR7136 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab128860 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
1/1000. Predicted molecular weight: 15 kDa.
For unpurified use at 1/1000 - 1/10000. |
Notes |
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WB
1/1000. Predicted molecular weight: 15 kDa. For unpurified use at 1/1000 - 1/10000. |
Target
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Function
FABP are thought to play a role in the intracellular transport of long-chain fatty acids and their acyl-CoA esters. FABP2 is probably involved in triglyceride-rich lipoprotein synthesis. Binds saturated long-chain fatty acids with a high affinity, but binds with a lower affinity to unsaturated long-chain fatty acids. FABP2 may also help maintain energy homeostasis by functioning as a lipid sensor. -
Tissue specificity
Expressed in the small intestine and at much lower levels in the large intestine. Highest expression levels in the jejunum. -
Sequence similarities
Belongs to the calycin superfamily. Fatty-acid binding protein (FABP) family. -
Domain
Forms a beta-barrel structure that accommodates the hydrophobic ligand in its interior. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 2169 Human
- Entrez Gene: 14079 Mouse
- Entrez Gene: 25598 Rat
- Omim: 134640 Human
- SwissProt: P12104 Human
- SwissProt: P55050 Mouse
- SwissProt: P02693 Rat
- Unigene: 282265 Human
see all -
Alternative names
- FABP 2 antibody
- FABP2 antibody
- FABPI antibody
see all
Images
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All lanes : Anti-I-FABP antibody [EPR7136] (ab128860) at 1/10000 dilution (Purified)
Lane 1 : Human small intestine lysates
Lane 2 : Mouse small intestine lysates
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Predicted band size: 15 kDa
Observed band size: 14 kDa why is the actual band size different from the predicted?Blocking/Diluting buffer: 5% NFDM/TBST
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Anti-I-FABP antibody [EPR7136] (ab128860) at 1/1000 dilution (Purified) + Rat small intestine lysates at 15 µg
Secondary
Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Predicted band size: 15 kDa
Observed band size: 14 kDa why is the actual band size different from the predicted?Blocking/Diluting buffer: 5% NFDM/TBST
Datasheets and documents
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SDS download
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Datasheet download
References (3)
ab128860 has been referenced in 3 publications.
- Chamseddine D et al. The mitochondrial UPR regulator ATF5 promotes intestinal barrier function via control of the satiety response. Cell Rep 41:111789 (2022). PubMed: 36516750
- Guo J et al. Metformin-Induced Changes of the Coding Transcriptome and Non-Coding RNAs in the Livers of Non-Alcoholic Fatty Liver Disease Mice. Cell Physiol Biochem 45:1487-1505 (2018). PubMed: 29466788
- Costa DK et al. Reduced intestinal lipid absorption and body weight-independent improvements in insulin sensitivity in high-fat diet-fed Park2 knockout mice. Am J Physiol Endocrinol Metab 311:E105-16 (2016). PubMed: 27166280