Anti-I-FABP antibody (ab60272)
Key features and details
- Goat polyclonal to I-FABP
- Suitable for: WB, ICC/IF, IHC-P, Flow Cyt, ELISA
- Reacts with: Cat, Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
-
Product name
Anti-I-FABP antibody
See all I-FABP primary antibodies -
Description
Goat polyclonal to I-FABP -
Host species
Goat -
Tested applications
Suitable for: WB, ICC/IF, IHC-P, Flow Cyt, ELISAmore details -
Species reactivity
Reacts with: Cat, Human
Predicted to work with: Rat, Horse, Chicken, Cow, Dog, Pig, Chimpanzee, Rhesus monkey -
Immunogen
Synthetic peptide corresponding to Human I-FABP aa 121-132 (C terminal).
Sequence:C-EGVEAKRIFKKD
(Peptide available asab115238) -
Positive control
- WB: Human duodenum and ileum lysates. IHC-P: Human small intestine tissue. Flow Cyt: A431 cells.
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA -
Concentration information loading...
-
Purity
Immunogen affinity purified -
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
-
Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab60272 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
Use a concentration of 0.001 - 0.003 µg/ml. Detects a band of approximately 16 kDa (predicted molecular weight: 15 kDa).
A 1 hour primary incubation is recommended for this product. |
|
ICC/IF |
Use at an assay dependent concentration. PubMed: 23236492
|
|
IHC-P | (1) |
Use a concentration of 2 - 3 µg/ml.
|
Flow Cyt |
Use a concentration of 10 µg/ml.
|
|
ELISA |
Use at an assay dependent concentration.
Antibody detection limit dilution 1:64000. |
Notes |
---|
WB
Use a concentration of 0.001 - 0.003 µg/ml. Detects a band of approximately 16 kDa (predicted molecular weight: 15 kDa). A 1 hour primary incubation is recommended for this product. |
ICC/IF
Use at an assay dependent concentration. PubMed: 23236492 |
IHC-P
Use a concentration of 2 - 3 µg/ml. |
Flow Cyt
Use a concentration of 10 µg/ml. |
ELISA
Use at an assay dependent concentration. Antibody detection limit dilution 1:64000. |
Target
-
Function
FABP are thought to play a role in the intracellular transport of long-chain fatty acids and their acyl-CoA esters. FABP2 is probably involved in triglyceride-rich lipoprotein synthesis. Binds saturated long-chain fatty acids with a high affinity, but binds with a lower affinity to unsaturated long-chain fatty acids. FABP2 may also help maintain energy homeostasis by functioning as a lipid sensor. -
Tissue specificity
Expressed in the small intestine and at much lower levels in the large intestine. Highest expression levels in the jejunum. -
Sequence similarities
Belongs to the calycin superfamily. Fatty-acid binding protein (FABP) family. -
Domain
Forms a beta-barrel structure that accommodates the hydrophobic ligand in its interior. -
Cellular localization
Cytoplasm. - Information by UniProt
-
Database links
- Entrez Gene: 2169 Human
- Entrez Gene: 25598 Rat
- Omim: 134640 Human
- SwissProt: Q56JX9 Cow
- SwissProt: P12104 Human
- SwissProt: Q45KW7 Pig
- SwissProt: Q56G53 Pig
- SwissProt: P02693 Rat
see all -
Alternative names
- FABP 2 antibody
- FABP2 antibody
- FABPI antibody
see all
Images
-
Lane 1 : Anti-I-FABP antibody (ab60272) at 0.001 µg/ml
Lane 2 : Anti-I-FABP antibody (ab60272) at 0.003 µg/ml
Lane 1 : Human Duodenum lysate in RIPA buffer
Lane 2 : Ileum lysate in RIPA buffer
Lysates/proteins at 35 µg per lane.
Predicted band size: 15 kDa
Observed band size: 15-16 kDa why is the actual band size different from the predicted?Detected by chemiluminescence.
-
ab60272 Flow cytometric analysis of paraformaldehyde fixed A431 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
-
ab60272 (2.5µg/ml) staining of paraffin embedded Human Small Intestine. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Protocols
Datasheets and documents
-
Datasheet download
References (5)
ab60272 has been referenced in 5 publications.
- Baumgarten HD et al. The EXTrauterine Environment for Neonatal Development Supports Normal Intestinal Maturation and Development. Cell Mol Gastroenterol Hepatol 10:623-637 (2020). PubMed: 32474164
- Reicher N et al. It's All About Timing: Early Feeding Promotes Intestinal Maturation by Shifting the Ratios of Specialized Epithelial Cells in Chicks. Front Physiol 11:596457 (2020). PubMed: 33391016
- Tian M et al. Fat Encapsulation Reduces Diarrhea in Piglets Partially by Repairing the Intestinal Barrier and Improving Fatty Acid Transport. Animals (Basel) 11:N/A (2020). PubMed: 33375218
- Ziegler AL et al. Epithelial restitution defect in neonatal jejunum is rescued by juvenile mucosal homogenate in a pig model of intestinal ischemic injury and repair. PLoS One 13:e0200674 (2018). PubMed: 30138372
- Treutlein G et al. Novel potential interacting partners of fibronectin in spontaneous animal model of interstitial cystitis. PLoS One 7:e51391 (2012). WB, ICC/IF ; Cat . PubMed: 23236492