Overview
Product nameintestinal FABP proteinSee all intestinal FABP proteins and peptides ...
Protein descriptionRecombinant Full length Human intestinal FABP (amino acids 2-131) with an N terminal His tag; 141 amino acids, MWt 16.32kDa (calculated). (Uniprot ID = P12104).
Uniprot accessionP12104
Molecular weight16.320kDa inclusive of tags
Protein length130 amino acids
Expression hostE. coli
Properties
Purity> 95
% by SDS-PAGE
Purification notesab115502 is purified using Ni-NTA chromatography.
FormLyophilised:Add deionized water to prepare a working stock solution of approximately 0.5 mg/mL and let the lyophilized pellet dissolve completely. Aliquot reconstituted protein to avoid repeated freezing/thawing cycles and store at –80°C for long term storage. Reconstituted protein can be stored at 4°C for a week.
Storage instructionsStore at -80°C
Storage bufferpH: 7.40
Constituents: 0.29% Sodium chloride, 0.69% Phosphate Buffer
Concentration information loading...
Sequence notesMKHHHHHHASAFDSTWKVDRSENYDKFMEKMGVNIVKRKL AAHDNLKLTITQEGNKFTVKESSAFRNIEVVFELGVTFNY NLADGTELRGTWSLEGNKLIGKFKRTDNGNELNTVREIIG DELVQTYVYEGVEAKRIFKKD
Research Areas
Applications
Our Abpromise guarantee covers the use of
ab115502
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|
Application
|
Notes |
| SDS-PAGE |
SDS-PAGE: Use at an assay dependent concentration. |
| WB |
WB: Use at an assay dependent dilution. |
| ELISA |
ELISA: Use at an assay dependent dilution. |
Application notesThis peptide can be used with studies using ab60272 and ab77386.
Protein info
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Alternative names
FABPI_HUMANFatty acid binding proteinFatty acid binding protein 2 intestinalFatty acid binding protein intestinalFatty acid-binding proteinFatty acid-binding protein 2I FABPI-FABPIFABPintestinalIntestinal fatty acid binding protein 2Intestinal-type fatty acid-binding proteinMGC133132OTTHUMP00000163925
see all
FunctionFABP are thought to play a role in the intracellular transport of long-chain fatty acids and their acyl-CoA esters. FABP2 is probably involved in triglyceride-rich lipoprotein synthesis. Binds saturated long-chain fatty acids with a high affinity, but binds with a lower affinity to unsaturated long-chain fatty acids. FABP2 may also help maintain energy homeostasis by functioning as a lipid sensor.
Tissue specificityExpressed in the small intestine and at much lower levels in the large intestine. Highest expression levels in the jejunum.
Sequence similaritiesBelongs to the calycin superfamily. Fatty-acid binding protein (FABP) family.
DomainForms a beta-barrel structure that accommodates the hydrophobic ligand in its interior.
Cellular localizationCytoplasm.
intestinal FABP protein images
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SDS-PAGE - intestinal FABP protein (ab115502)
14% SDS-PAGE showing ab115502
Lane 1: M.W. marker – 14, 21, 31, 45, 66, 97 kDa
Lane 2: reduced and boiled sample, 5µg/lane.
Lane 3: non-reduced and non-boiled sample, 5µg/lane.
References for intestinal FABP protein (ab115502)
ab115502
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
