Recombinant
RabMAb

Anti-intestinal alkaline phosphatase antibody [EPR15201(B)] (ab186422)

Overview

  • Product name
    Anti-intestinal alkaline phosphatase antibody [EPR15201(B)]
    See all intestinal alkaline phosphatase primary antibodies
  • Description
    Rabbit monoclonal [EPR15201(B)] to intestinal alkaline phosphatase
  • Specificity
    The immunogen used for this product shares 85.7% homology with ALPPL2 (Alkaline phosphatase placental-like). Cross-reactivity with this protein has not been confirmed experimentally.
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human intestinal alkaline phosphatase aa 1-100. The exact sequence is proprietary.
    Database link: P09923

  • Positive control
    • HeLa, JAR and Human placenta lysate; Human small intestine tissue.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Applications

Our Abpromise guarantee covers the use of ab186422 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 68 kDa (predicted molecular weight: 57 kDa).
IHC-P 1/500.

Target

  • Sequence similarities
    Belongs to the alkaline phosphatase family.
  • Cellular localization
    Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Alkaline phosphatase intestinal antibody
    • Alkaline phosphomonoesterase antibody
    • Alpi antibody
    • Glycerophosphatase antibody
    • IAP antibody
    • Intestinal alkaline phosphatase antibody
    • Intestinal-type alkaline phosphatase antibody
    • Kasahara isozyme antibody
    • OTTHUMP00000164357 antibody
    • PPBI_HUMAN antibody
    see all

Images

  • All lanes : Anti-intestinal alkaline phosphatase antibody [EPR15201(B)] (ab186422) at 1/1000 dilution

    Lane 1 : Caco-2 (Human colorectal adenocarcinoma epithelial cell) whole cell lysates
    Lane 2 : Human placenta lysates

    Lysates/proteins at 15 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size : 57 kDa
    Observed band size : 57 kDa


    Exposure time : 15 seconds

    Blocking and Diluting buffer: 5% NFDM/TBST

    This antibody does not work in Caco-2 cells which should be positive (PMID: 20660763).

  • Immunohistochemical analysis of paraffin-embedded Human small intestine tissue labeling Alkaline Phosphatase with ab186422 at 1/500 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.

  • Anti-intestinal alkaline phosphatase antibody [EPR15201(B)] (ab186422) at 1/10000 dilution + HeLa cell lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size : 57 kDa
  • All lanes : Anti-intestinal alkaline phosphatase antibody [EPR15201(B)] (ab186422) at 1/1000 dilution

    Lane 1 : JAR cell lysate
    Lane 2 : Human placenta lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size : 57 kDa

References

This product has been referenced in:
  • Ning S  et al. Genetic differences in osteogenic differentiation potency in the thoracic ossification of the ligamentum flavum under cyclic mechanical stress. Int J Mol Med 39:135-143 (2017). Read more (PubMed: 28004120) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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