Recombinant Anti-intestinal alkaline phosphatase antibody [EPR15201(B)] (ab186422)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR15201(B)] to intestinal alkaline phosphatase
- Suitable for: WB, IHC-P
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-intestinal alkaline phosphatase antibody [EPR15201(B)]
See all intestinal alkaline phosphatase primary antibodies -
Description
Rabbit monoclonal [EPR15201(B)] to intestinal alkaline phosphatase -
Host species
Rabbit -
Specificity
The immunogen used for this product shares 85.7% homology with ALPPL2 (Alkaline phosphatase placental-like). Cross-reactivity with this protein has not been confirmed experimentally. -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- HeLa, JAR and Human placenta lysate; Human small intestine tissue.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR15201(B) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab186422 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000 - 1/10000. Detects a band of approximately 68 kDa (predicted molecular weight: 57 kDa).
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IHC-P |
1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Notes |
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WB
1/1000 - 1/10000. Detects a band of approximately 68 kDa (predicted molecular weight: 57 kDa). |
IHC-P
1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Target
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Sequence similarities
Belongs to the alkaline phosphatase family. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 248 Human
- Omim: 171740 Human
- SwissProt: P09923 Human
- Unigene: 284255 Human
- Unigene: 37009 Human
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Alternative names
- Alkaline phosphatase intestinal antibody
- Alkaline phosphomonoesterase antibody
- Alpi antibody
see all
Images
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All lanes : Anti-intestinal alkaline phosphatase antibody [EPR15201(B)] (ab186422) at 1/1000 dilution
Lane 1 : Caco-2 (Human colorectal adenocarcinoma epithelial cell) whole cell lysates
Lane 2 : Human placenta lysates
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 57 kDa
Observed band size: 57 kDa
Exposure time: 15 secondsBlocking and Diluting buffer: 5% NFDM/TBST
This antibody does not work in Caco-2 cells which should be positive (PMID: 20660763).
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Immunohistochemical analysis of paraffin-embedded Human small intestine tissue labeling Alkaline Phosphatase with ab186422 at 1/500 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.
Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
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Anti-intestinal alkaline phosphatase antibody [EPR15201(B)] (ab186422) at 1/10000 dilution + HeLa cell lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Predicted band size: 57 kDa -
All lanes : Anti-intestinal alkaline phosphatase antibody [EPR15201(B)] (ab186422) at 1/1000 dilution
Lane 1 : JAR cell lysate
Lane 2 : Human placenta lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Predicted band size: 57 kDa
Protocols
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (6)
ab186422 has been referenced in 6 publications.
- Takeuchi A et al. IIAEK Targets Intestinal Alkaline Phosphatase (IAP) to Improve Cholesterol Metabolism with a Specific Activation of IAP and Downregulation of ABCA1. Nutrients 12:N/A (2020). PubMed: 32961978
- Good C et al. Type III interferon signaling restricts enterovirus 71 infection of goblet cells. Sci Adv 5:eaau4255 (2019). PubMed: 30854425
- Bing T et al. Aptameric Probe Specifically Binding Protein Heterodimer Rather Than Monomers. Adv Sci (Weinh) 6:1900143 (2019). PubMed: 31179220
- Zhou D et al. Valproic acid prevents glucocorticoid-induced osteonecrosis of the femoral head of rats. Int J Mol Med 41:3433-3447 (2018). PubMed: 29512684
- Ning S et al. Genetic differences in osteogenic differentiation potency in the thoracic ossification of the ligamentum flavum under cyclic mechanical stress. Int J Mol Med 39:135-143 (2017). PubMed: 28004120
- Cui Y et al. Silencing of Long Non-Coding RNA NONHSAT009968 Ameliorates the Staphylococcal Protein A-Inhibited Osteogenic Differentiation in Human Bone Mesenchymal Stem Cells. Cell Physiol Biochem 39:1347-59 (2016). PubMed: 27607236