Anti-intestinal FABP antibody [9A9B7B3] (ab109772)

Overview

  • Product nameAnti-intestinal FABP antibody [9A9B7B3]
    See all intestinal FABP primary antibodies
  • Description
    Mouse monoclonal [9A9B7B3] to intestinal FABP
  • Tested applicationsWB, ELISA, IHC-P, Flow Cyt, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant Human intestinal intestinal FABP.

  • Positive control
    • Human Small Intestine tissue; LOVO cell lysate, intestinal FABP-transfected HEK293 cell lysate; LOVO and 3T3-L1 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab109772 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/2000. Predicted molecular weight: 15 kDa.
ELISA Use at an assay dependent dilution.
IHC-P 1/100.
Flow Cyt 1/200 - 1/400.
ICC/IF 1/200 - 1/1000.

Target

  • FunctionFABP are thought to play a role in the intracellular transport of long-chain fatty acids and their acyl-CoA esters. FABP2 is probably involved in triglyceride-rich lipoprotein synthesis. Binds saturated long-chain fatty acids with a high affinity, but binds with a lower affinity to unsaturated long-chain fatty acids. FABP2 may also help maintain energy homeostasis by functioning as a lipid sensor.
  • Tissue specificityExpressed in the small intestine and at much lower levels in the large intestine. Highest expression levels in the jejunum.
  • Sequence similaritiesBelongs to the calycin superfamily. Fatty-acid binding protein (FABP) family.
  • DomainForms a beta-barrel structure that accommodates the hydrophobic ligand in its interior.
  • Cellular localizationCytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • FABP 2 antibody
    • FABP2 antibody
    • FABPI antibody
    • FABPI_HUMAN antibody
    • Fatty acid binding protein 2 intestinal antibody
    • Fatty acid binding protein antibody
    • Fatty acid binding protein intestinal antibody
    • Fatty acid-binding protein 2 antibody
    • Fatty acid-binding protein antibody
    • I FABP antibody
    • I-FABP antibody
    • IFABP antibody
    • intestinal antibody
    • Intestinal fatty acid binding protein 2 antibody
    • Intestinal-type fatty acid-binding protein antibody
    • MGC133132 antibody
    • OTTHUMP00000163925 antibody
    see all

Anti-intestinal FABP antibody [9A9B7B3] images

  • All lanes : Anti-intestinal FABP antibody [9A9B7B3] (ab109772) at 1/500 dilution

    Lane 1 : intestinal FABP-hIgGFc transfected HEK293 cell lysate
    Lane 2 : LOVO cell lysate


    Predicted band size : 15 kDa
  • Flow cytometric analysis of LOVO cells using ab109772 at 1/200 dilution (green) or a negative control (purple).
  • Immunofluorescence analysis of intestinal FABP in 3T3-L1 cells using ab109772 at 1/200 dilution (green).
    Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
  • Immunohistological staining of Human small intestine, Formalin-Fixed, Paraffin-Embedded tissue using ab109772 at 1/100 dilution.

References for Anti-intestinal FABP antibody [9A9B7B3] (ab109772)

ab109772 has not yet been referenced specifically in any publications.

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