Overview

  • Product name
    Anti-intestinal FABP antibody
    See all intestinal FABP primary antibodies
  • Description
    Rabbit polyclonal to intestinal FABP
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Rat, Horse, Chicken, Cow, Pig
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 100 to the C-terminus of Human intestinal FABP.

    (Peptide available as ab88236.)

  • Positive control
    • This antibody gave a positive signal in the following whole cell lysates: Caco2; SW480; LoVo.

Properties

Applications

Our Abpromise guarantee covers the use of ab77386 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 16 kDa (predicted molecular weight: 15 kDa).

Target

  • Function
    FABP are thought to play a role in the intracellular transport of long-chain fatty acids and their acyl-CoA esters. FABP2 is probably involved in triglyceride-rich lipoprotein synthesis. Binds saturated long-chain fatty acids with a high affinity, but binds with a lower affinity to unsaturated long-chain fatty acids. FABP2 may also help maintain energy homeostasis by functioning as a lipid sensor.
  • Tissue specificity
    Expressed in the small intestine and at much lower levels in the large intestine. Highest expression levels in the jejunum.
  • Sequence similarities
    Belongs to the calycin superfamily. Fatty-acid binding protein (FABP) family.
  • Domain
    Forms a beta-barrel structure that accommodates the hydrophobic ligand in its interior.
  • Cellular localization
    Cytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • FABP 2 antibody
    • FABP2 antibody
    • FABPI antibody
    • FABPI_HUMAN antibody
    • Fatty acid binding protein 2 intestinal antibody
    • Fatty acid binding protein antibody
    • Fatty acid binding protein intestinal antibody
    • Fatty acid-binding protein 2 antibody
    • Fatty acid-binding protein antibody
    • I FABP antibody
    • I-FABP antibody
    • IFABP antibody
    • intestinal antibody
    • Intestinal fatty acid binding protein 2 antibody
    • Intestinal-type fatty acid-binding protein antibody
    • MGC133132 antibody
    • OTTHUMP00000163925 antibody
    see all

Images

  • All lanes : Anti-intestinal FABP antibody (ab77386) at 1 µg/ml

    Lane 1 : Caco 2 (Human colonic carcinoma cell line) Whole Cell Lysate
    Lane 2 : SW480 (Human colon adenocarcinoma cell line) Whole Cell Lysate
    Lane 3 : LOVO (Human colon adenocarcinoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 15 kDa
    Observed band size : 15 kDa
    Additional bands at : 115 kDa,80 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 12 minutes

References

ab77386 has not yet been referenced specifically in any publications.

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There are currently no Customer reviews or Questions for ab77386.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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