Anti-Intestine specific homeobox antibody (ab82787)

Overview

  • Product nameAnti-Intestine specific homeobox antibody
  • Description
    Rabbit polyclonal to Intestine specific homeobox
  • Tested applicationsSuitable for: ELISA, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to a region within N terminal amino acids 36-85 (ILKRPARRSD MDRPEGPGEE GPGEAAASGS GLEKPPKDQP QEGRKSKRRV) of Human Intestine specific homeobox (NP_001008494).

  • Positive control
    • 721_B cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab82787 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay: 1/312500.

WB Use a concentration of 1 µg/ml. Predicted molecular weight: 27 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • FunctionTranscription factor that regulates gene expression in intestine. May participate in vitamin A metabolism most likely by regulating BCMO1 expression in the intestine.
  • Sequence similaritiesContains 1 homeobox DNA-binding domain.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • DKFZp781N2395 antibody
    • Intestine-specific homeobox antibody
    • Isx antibody
    • ISX_HUMAN antibody
    • MGC138417 antibody
    • OTTHUMP00000028558 antibody
    • Pancreas intestine homeodomain transcription factor antibody
    • Pix 1 antibody
    • RAX like homeobox antibody
    • RAX-like homeobox antibody
    • RAXLX antibody
    see all

Anti-Intestine specific homeobox antibody images

  • Anti-Intestine specific homeobox antibody (ab82787) at 1 µg/ml (in 5% skim milk / PBS buffer) + 721_B cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 27 kDa
    Observed band size : 31 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 64 kDa. We are unsure as to the identity of these extra bands.

References for Anti-Intestine specific homeobox antibody (ab82787)

ab82787 has not yet been referenced specifically in any publications.

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