Anti-Intra Acrosomal Protein antibody (ab81229)

Overview

  • Product nameAnti-Intra Acrosomal Protein antibody
    See all Intra Acrosomal Protein primary antibodies
  • Description
    Rabbit polyclonal to Intra Acrosomal Protein
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Rabbit
  • Immunogen

    Synthetic peptide corresponding to a region within N terminal amino acids 2-51 (NRFLLLMSLY LLGSARGTSS QPNELSGSID HQTSVQQLPG EFFSLENPSD) of Human Intra Acrosomal Protein (NP_001603).

  • Positive control
    • Human fetal liver lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab81229 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 29 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

Titre using peptide based assay: 1/62500.

Target

  • Tissue specificityTestis.
  • Cellular localizationCytoplasmic vesicle > secretory vesicle > acrosome. Nascent acrosomal vesicle of Golgi phase spermatids.
  • Information by UniProt
  • Database links
  • Alternative names
    • Acrosomal protein SP 10 antibody
    • Acrosomal protein SP-10 antibody
    • Acrosomal vesicle protein 1 antibody
    • ACRV1 antibody
    • ASPX_HUMAN antibody
    • D11S4365 antibody
    • Msa63 antibody
    • SP 10 antibody
    • Sp10 antibody
    • SPACA2 antibody
    • Sperm protein 10 antibody
    see all

Anti-Intra Acrosomal Protein antibody images

  • Anti-Intra Acrosomal Protein antibody (ab81229) at 1 µg/ml (in 5% skim milk / PBS buffer) + Human fetal liver lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 29 kDa
    Observed band size : 33 kDa (why is the actual band size different from the predicted?)

References for Anti-Intra Acrosomal Protein antibody (ab81229)

ab81229 has not yet been referenced specifically in any publications.

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