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Tissue/ cell preparation (Dog). Freshly ejaculated dog sperms were washed in PBS and extracted in 3% acetic acid, 10% glycerol, 30 mM benzaminidine. The acid extract was dialyzed against 0.2% acetic acid, lyophylized and subsequently used for immunization.
The antibody reacts with intra-acrosomal proteins (92 and 42 kDa) of canine sperms.
Our Abpromise guarantee covers the use of ab14300 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent concentration. Predicted molecular weight: 32 kDa.|
|ICC||Use at an assay dependent concentration.
Membrane permeabilization (acetone) essential.
ab14300 has not yet been referenced specifically in any publications.