This fast track antibody is not yet fully characterized. It is subject to these terms and conditions

Overview

Properties

Associated products

Target

  • RelevanceIntrinsic factor promotes absorption of the essential vitamin Cobalamin (Cbl) in the ileum by specific receptor mediated endocytosis. Defects in the gene GIF are the cause of hereditary intrinsic factor deficiency (IFD) also called congenital pernicious anemia. IFD is an autosomal recessive disorder characterized by megaloblastic anemia.
  • Cellular localizationSecreted
  • Database links
  • Alternative names
    • Gastric Intrinsic Factor antibody
    • Gastric intrinsic factor (vitamin B synthesis) antibody
    • GIF antibody
    • IF antibody
    • IFMH antibody
    • INF antibody
    • Intrinsic factor antibody
    • TCM3 antibody
    • TCN3 antibody
    see all

Anti-Intrinsic Factor antibody images

This Fast-Track antibody is not yet fully characterised. These images represent inconclusive preliminary data.

  • Anti-Intrinsic Factor antibody (ab91322) at 1 µg/ml + Human Stomach Tumour at 10 µg

    Secondary
    Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Observed band size : 55 kDa (why is the actual band size different from the predicted?)


    Exposure time : 10 minutes
  • ICC/IF image of ab91322 stained MCF7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab91322, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-Intrinsic Factor antibody (ab91322)

ab91322 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"