The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesIP: Use at 1-4µg/ml lysate.
WB: 1/1000 - 1/5000. Detects a band of approximately 118 kDa (predicted molecular weight: 118 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
RelevanceFunction: Component of the Integrator complex. The Integrator complex is involved in the small nuclear RNAs (snRNA) U1 and U2 transcription and in their 3'-box-dependent processing. The Integrator complex is associated with the C-terminal domain (CTD) of RNA polymerase II largest subunit (POLR2A) and is recruited to the U1 and U2 snRNAs genes.
Component of the SOSS complex, a multiprotein complex that functions downstream of the MRN complex to promote DNA repair and G2/M checkpoint. The SOSS complex associates with single-stranded DNA at DNA lesions and influences diverse endpoints in the cellular DNA damage response including cell-cycle checkpoint activation, recombinational repair and maintenance of genomic stability. The SOSS complex is required for efficient homologous recombination-dependent repair of double-strand breaks (DSBs) and ATM-dependent signaling pathways. In the SOSS complex, it is required for the assembly of the complex and for stabilization of the complex at DNA damage sites.
Similarity: Belongs to the Integrator subunit 3 family.