Overview

  • Product name
  • Description
    Rabbit polyclonal to INTS5
  • Tested applications
    Suitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Horse, Chimpanzee, Ferret, Rhesus monkey, Gorilla, Chinese hamster
  • Immunogen

    Synthetic peptide corresponding to a region between residues 1000 and 1050 of human INTS5 (NP_085131.1)

  • Positive control
    • HeLa and NIH3T3 whole cell lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab74405 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 108 kDa.
IP Use at 2-5 µg/mg of lysate.

Target

  • Relevance
    INTS5 is a subunit of the Integrator complex, a complex involved in the small nuclear RNAs (snRNA) U1 and U2 transcription and in their 3'-box-dependent processing. The Integrator complex is associated with the C-terminal domain (CTD) of RNA polymerase II largest subunit (POLR2A) and is recruited to the U1 and U2 snRNAs genes.
  • Cellular localization
    Nucleus. Membrane; Multi pass membrane protein.
  • Database links
  • Alternative names
    • 1110055N21Rik antibody
    • INT5 antibody
    • integrator complex subunit 5 antibody
    • KIAA1698 antibody
    • mKIAA1698 antibody
    • RGD1309370 antibody
    see all

Images

  • All lanes : Anti-INTS5 antibody (ab74405) at 0.4 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : NIH3T3 whole cell lysate at 50 µg

    Developed using the ECL technique

    Predicted band size : 108 kDa
    Observed band size : 108 kDa
    Additional bands at : 75 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 10 seconds
  • Detection of INTS5 by Western Blot of Immunprecipitate.
    ab74405, at 1µg/ml, staining INTS5 in HeLa whole cell lysate immunoprecipitated using ab74405 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
    Detection: Chemiluminescence with exposure time of 30 seconds.

References

This product has been referenced in:
  • Oegema R  et al. Human mutations in integrator complex subunits link transcriptome integrity to brain development. PLoS Genet 13:e1006809 (2017). WB ; Human . Read more (PubMed: 28542170) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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