Purification notesThis product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process including delipidation, salt fractionation, ion exchange chromatography and extensive dialysis against buffer.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesDot: Use at an assay dependent dilution.
ELISA: 1/4000 - 1/20000.
This product has been assayed against 1.0µg of Invertase in a standard capture ELISA using Peroxidase conjugated streptavidin and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic-acid]) as a substrate for 30 minutes at RT.
IM: Use at an assay dependent dilution.
WB: Use at an assay dependent dilution. Predicted molecular weight: 52 kDa.
Suitable for other antibody based assays using streptavidin or avidin conjugates.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
RelevanceInvertase hydrolyses the terminal non reducing beta D fructofuranoside residues in beta D fructofuranosides. It is the enzyme that bees use to convert nectar into honey. Industrial and confectionary applications use yeast invertase to split sucrose into fructose and glucose and also to improve the shelf life of food products.
Beta fructofuranosidase 2 antibody
References for Anti-Invertase antibody (Biotin) (ab34530)
has not yet been referenced specifically in any publications.
Publishing research using ab34530? Please let us know so that we can cite the reference in this datasheet.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"