Overview

  • Product nameAnti-Involucrin antibody [0.N.341]
    See all Involucrin primary antibodies
  • Description
    Mouse monoclonal [0.N.341] to Involucrin
  • SpecificityRecognizes involucrin at 66-170kD, depending on cell type. Stains involucrin in a variety of sizes: 170kD in MCF7 cells, a doublet of ~115kD and 150kD in gorilla and owl monkey, 66kD in canine and a doublet of 105kD in porcine.
  • Tested applicationsSuitable for: WB, IP, ICC/IF, IHC-P, ELISAmore details
  • Species reactivity
    Reacts with: Rat, Dog, Human, Pig, Monkey, Owl, Gorilla
  • Immunogen

    Full length native protein (purified) (Human).

  • EpitopeThe epitope maps between residues 421-568 of human involucrin.
  • Positive control
    • LS174T cells and skin

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Add glycerol to a final volume of 50% for extra stability and aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 0.09% Sodium Azide
    Constituents: 0.2% BSA, 10mM PBS, pH 7.4
  • Concentration information loading...
  • PurityProtein G purified
  • ClonalityMonoclonal
  • Clone number0.N.341
  • IsotypeIgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab14504 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
IP
ICC/IF
IHC-P
ELISA
  • Application notesELISA: Use at an assay dependent dilution.
    IF: Use at an assay dependent dilution.
    IHC-P: Use at a concentration of 1 - 2 µg/ml. Perform enzymatic antigen retrieval before commencing with IHC staining protocol. For staining formalin-fixed tissues, digest sections with trypsin at 1mg/ml PBS, for 10 min at 37°C. Note: Enzyme digestion is better than citrate buffer based epitope unmasking.
    IP: Use 2 µg/mg protein lysate (Denatured verified; Use Protein G).
    WB: Use at a concentration of 1 - 2 µg/ml. (Human form at 170 kDa; see under "Specificity" for other species molecular weights.)

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    References for Anti-Involucrin antibody [0.N.341] (ab14504)

    This product has been referenced in:
    • Berge U  et al. Hormetic modulation of differentiation of normal human epidermal keratinocytes undergoing replicative senescence in vitro. Exp Gerontol 43:658-62 (2008). ELISA ; Human . Read more (PubMed: 18262743) »
    • Berge U  et al. Sugar-induced premature aging and altered differentiation in human epidermal keratinocytes. Ann N Y Acad Sci 1100:524-9 (2007). Read more (PubMed: 17460218) »

    See all 3 Publications for this product

    Product Wall

    There are currently no Abreviews or Questions for ab14504.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"