SpecificityRecognizes involucrin at 66-170kD, depending on cell type. Stains involucrin in a variety of sizes: 170kD in MCF7 cells, a doublet of ~115kD and 150kD in gorilla and owl monkey, 66kD in canine and a doublet of 105kD in porcine.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesELISA: Use at an assay dependent dilution.
IF: Use at an assay dependent dilution.
IHC-P: Use at a concentration of 1 - 2 µg/ml. Perform enzymatic antigen retrieval before commencing with IHC staining protocol. For staining formalin-fixed tissues, digest sections with trypsin at 1mg/ml PBS, for 10 min at 37°C. Note: Enzyme digestion is better than citrate buffer based epitope unmasking.
IP: Use 2 µg/mg protein lysate (Denatured verified; Use Protein G).
WB: Use at a concentration of 1 - 2 µg/ml. (Human form at 170 kDa; see under "Specificity" for other species molecular weights.)
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionPart of the insoluble cornified cell envelope (CE) of stratified squamous epithelia.
Tissue specificityKeratinocytes of epidermis and other stratified squamous epithelia.
Sequence similaritiesBelongs to the involucrin family.
Post-translational modificationsSubstrate of transglutaminase. Some glutamines and lysines are cross-linked to other involucrin molecules, to other proteins such as keratin, desmoplakin, periplakin and envoplakin, and to lipids like omega-hydroxyceramide.
Cellular localizationCytoplasm. Constituent of the scaffolding of the cornified envelope.
References for Anti-Involucrin antibody [0.N.341] (ab14504)
This product has been referenced in:
Berge U et al. Hormetic modulation of differentiation of normal human epidermal keratinocytes undergoing replicative senescence in vitro. Exp Gerontol43:658-62 (2008).
Read more (PubMed: 18262743) »
Berge U et al. Sugar-induced premature aging and altered differentiation in human epidermal keratinocytes. Ann N Y Acad Sci1100:524-9 (2007).
Read more (PubMed: 17460218) »