This antibody gave a positive signal in Human Skin tissue lysate as well as the following whole cell lysates: Human Foreskin Fibroblast; HUVEC; HeLa; A431; Saos-2.
This antibody gave a positive result in IF in the following Formaldehyde fixed cell line: HeLa and MCF-7.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40 Preservative: 0.02% Sodium azide Constituent: PBS Note: Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml.
Use a concentration of 1 µg/ml. Detects a band of approximately 68 kDa (predicted molecular weight: 68 kDa).
FunctionPart of the insoluble cornified cell envelope (CE) of stratified squamous epithelia.
Tissue specificityKeratinocytes of epidermis and other stratified squamous epithelia.
Sequence similaritiesBelongs to the involucrin family.
Post-translational modificationsSubstrate of transglutaminase. Some glutamines and lysines are cross-linked to other involucrin molecules, to other proteins such as keratin, desmoplakin, periplakin and envoplakin, and to lipids like omega-hydroxyceramide.
Cellular localizationCytoplasm. Constituent of the scaffolding of the cornified envelope.
ICC/IF image of ab111781 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab111781 at 1µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in formaldehyde fixed (4%, 10min) MCF-7 cells.
References for Anti-Involucrin antibody (ab111781)
has not yet been referenced specifically in any publications.
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