The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 68 kDa.
FunctionPart of the insoluble cornified cell envelope (CE) of stratified squamous epithelia.
Tissue specificityKeratinocytes of epidermis and other stratified squamous epithelia.
Sequence similaritiesBelongs to the involucrin family.
Post-translational modificationsSubstrate of transglutaminase. Some glutamines and lysines are cross-linked to other involucrin molecules, to other proteins such as keratin, desmoplakin, periplakin and envoplakin, and to lipids like omega-hydroxyceramide.
Cellular localizationCytoplasm. Constituent of the scaffolding of the cornified envelope.
Paraffin embedded formalin fixed human skin tissue section stained with anti Involucrin (ab27495).
References for Anti-Involucrin antibody (ab27495)
This product has been referenced in:
Melo SP et al. Somatic correction of junctional epidermolysis bullosa by a highly recombinogenic AAV variant. Mol Ther22:725-33 (2014).
Read more (PubMed: 24390279) »
Lamb R & Ambler CA Keratinocytes propagated in serum-free, feeder-free culture conditions fail to form stratified epidermis in a reconstituted skin model. PLoS One8:e52494 (2013).
Read more (PubMed: 23326335) »