- Datasheet
- Specific References (3)
- Protocols
Overview
- Product nameAnti-Involucrin antibody
See all Involucrin primary antibodies - DescriptionRabbit polyclonal to Involucrin
- Specificityab53112 detects endogenous levels of total Involucrin protein.
- Tested applicationsSuitable for: ICC/IF, WB, IHC-P, ELISAmore details
- Species reactivityReacts with: Human
- Immunogen
A synthetic peptide derived from human Involucrin.
- Positive control
- Human breast carcinoma tissue and extracts from HuvEc cells.
Properties
- FormLiquid
- Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
- Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4 -
Concentration information loading... - PurityImmunogen affinity purified
- Purification notesab53112 was affinity purified from rabbit antiserum by affinity chromatography using epitope-specific immunogen.
- ClonalityPolyclonal
- IsotypeIgG
- Research areas
Associated products
- Compatible Secondaries
Applications
Our Abpromise guarantee covers the use of ab53112 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | Use at an assay dependent concentration. PubMed: 21067603 | |
| WB | 1/300 - 1/1000. Detects a band of approximately 68 kDa (predicted molecular weight: 68 kDa). | |
| IHC-P | Use at an assay dependent concentration. | |
| ELISA | 1/20000. |
Target
- FunctionPart of the insoluble cornified cell envelope (CE) of stratified squamous epithelia.
- Tissue specificityKeratinocytes of epidermis and other stratified squamous epithelia.
- Sequence similaritiesBelongs to the involucrin family.
- Post-translational
modificationsSubstrate of transglutaminase. Some glutamines and lysines are cross-linked to other involucrin molecules, to other proteins such as keratin, desmoplakin, periplakin and envoplakin, and to lipids like omega-hydroxyceramide. - Cellular localizationCytoplasm. Constituent of the scaffolding of the cornified envelope.
- Information by UniProt
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Database links
- Entrez Gene: 3713 Human
- Omim: 147360 Human
- SwissProt: P07476 Human
- Unigene: 516439 Human
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Alternative names
- INVO_HUMAN antibody
- Involucrin antibody
- IVL antibody
Anti-Involucrin antibody images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Involucrin antibody (ab53112)This image is courtesy of an Abreview submitted by Carl Hobbsab53112 staining human skin sections by IHC-P. The tissue was fixed with formaldehyde and a heat mediated antigen retrival step was performed with citric acid pH 6. Blocking of the sample was done with 1% BSA for 10 minutes at 21°C, followed by staining with ab53112 at 1/150 in TBS/BSA/azide for 16h at 21°C. A biotinylated goat anti-rabbit polyclonal antibody at 1/200 was used as the secondary antibody.
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Western blot - Involucrin antibody (ab53112)All lanes : Anti-Involucrin antibody (ab53112) at 1/300 dilution
Lane 1 : HuvEc cell extract
Lane 2 : HuvEc cell extract with immunising peptide
Predicted band size : 68 kDa
Observed band size : 68 kDa -
Immunocytochemistry/ Immunofluorescence - Anti-Involucrin antibody (ab53112)Image from DeRouen MC et al., BMC Dev Biol. 2010 Nov 10;10:112. Fig 1.; doi:10.1186/1471-213X-10-112; 10 November 2010, BMC Developmental Biology 2010, 10:112Immunofluorescence analysis of Human skin xenografts carrying either control GFP (left) or SHH retrovirus (right), staining Involucrin (red) with ab53112. -
Immunohistochemistry (Paraffin-embedded sections) - Involucrin antibody (ab53112)ab53112 at 1/50 dilution staining Involucrin in human breast carcinoma by Immunohistochemistry, Paraffin embedded tissue, in the absence and presence of the immunising peptide.ab53112 at 1/50 dilution staining Involucrin in human breast carcinoma by Immunohistochemistry, Paraffin embedded tissue, in the absence and presence of the immunising peptide.
References for Anti-Involucrin antibody (ab53112)
This product has been referenced in:
- Varone A et al. Endogenous two-photon fluorescence imaging elucidates metabolic changes related to enhanced glycolysis and glutamine consumption in precancerous epithelial tissues. Cancer Res 74:3067-75 (2014). Read more (PubMed: 24686167) »
- Ichida JK et al. Notch inhibition allows oncogene-independent generation of iPS cells. Nat Chem Biol 10:632-9 (2014). Read more (PubMed: 24952596) »
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"