A synthetic peptide derived from human Involucrin.
Our Abpromise guarantee covers the use of ab53112 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use at an assay dependent concentration. PubMed: 21067603|
|WB||1/300 - 1/1000. Detects a band of approximately 68 kDa (predicted molecular weight: 68 kDa).|
|IHC-P||Use at an assay dependent concentration.|
ab53112 staining human skin sections by IHC-P. The tissue was fixed with formaldehyde and a heat mediated antigen retrival step was performed with citric acid pH 6. Blocking of the sample was done with 1% BSA for 10 minutes at 21°C, followed by staining with ab53112 at 1/150 in TBS/BSA/azide for 16h at 21°C. A biotinylated goat anti-rabbit polyclonal antibody at 1/200 was used as the secondary antibody.