Customer reviews and FAQs

Overview

  • Product name
    Anti-Involucrin antibody
    See all Involucrin primary antibodies
  • Description
    Rabbit polyclonal to Involucrin
  • Specificity
    ab53112 detects endogenous levels of total Involucrin protein.
  • Tested applications
    Suitable for: ICC/IF, WB, IHC-P, ELISAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    A synthetic peptide derived from human Involucrin.

  • Positive control
    • Human breast carcinoma tissue and extracts from HuvEc cells.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    ab53112 was affinity purified from rabbit antiserum by affinity chromatography using epitope-specific immunogen.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab53112 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration. PubMed: 21067603
WB 1/300 - 1/1000. Detects a band of approximately 68 kDa (predicted molecular weight: 68 kDa).
IHC-P Use at an assay dependent concentration.
ELISA 1/20000.

Target

  • Function
    Part of the insoluble cornified cell envelope (CE) of stratified squamous epithelia.
  • Tissue specificity
    Keratinocytes of epidermis and other stratified squamous epithelia.
  • Sequence similarities
    Belongs to the involucrin family.
  • Post-translational
    modifications
    Substrate of transglutaminase. Some glutamines and lysines are cross-linked to other involucrin molecules, to other proteins such as keratin, desmoplakin, periplakin and envoplakin, and to lipids like omega-hydroxyceramide.
  • Cellular localization
    Cytoplasm. Constituent of the scaffolding of the cornified envelope.
  • Information by UniProt
  • Database links
    • Alternative names
      • INVO_HUMAN antibody
      • Involucrin antibody
      • IVL antibody

    Anti-Involucrin antibody images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Involucrin antibody (ab53112)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Involucrin antibody (ab53112)This image is courtesy of an Abreview submitted by Carl Hobbs

      ab53112 staining human skin sections by IHC-P. The tissue was fixed with formaldehyde and a heat mediated antigen retrival step was performed with citric acid pH 6. Blocking of the sample was done with 1% BSA for 10 minutes at 21°C, followed by staining with ab53112 at 1/150 in TBS/BSA/azide for 16h at 21°C. A biotinylated goat anti-rabbit polyclonal antibody at 1/200 was used as the secondary antibody.

      See Abreview

    • Western blot - Involucrin antibody (ab53112)
      Western blot - Involucrin antibody (ab53112)
      All lanes : Anti-Involucrin antibody (ab53112) at 1/300 dilution

      Lane 1 : HuvEc cell extract
      Lane 2 : HuvEc cell extract with immunising peptide


      Predicted band size : 68 kDa
      Observed band size : 68 kDa
    • Immunocytochemistry/ Immunofluorescence - Anti-Involucrin antibody (ab53112)
      Immunocytochemistry/ Immunofluorescence - Anti-Involucrin antibody (ab53112)Image from DeRouen MC et al., BMC Dev Biol. 2010 Nov 10;10:112. Fig 1.; doi:10.1186/1471-213X-10-112; 10 November 2010, BMC Developmental Biology 2010, 10:112
      Immunofluorescence analysis of Human skin xenografts carrying either control GFP (left) or SHH retrovirus (right), staining Involucrin (red) with ab53112.
    • Immunohistochemistry (Paraffin-embedded sections) - Involucrin antibody (ab53112)
      Immunohistochemistry (Paraffin-embedded sections) - Involucrin antibody (ab53112)
      ab53112 at 1/50 dilution staining Involucrin in human breast carcinoma by Immunohistochemistry, Paraffin embedded tissue, in the absence and presence of the immunising peptide.ab53112 at 1/50 dilution staining Involucrin in human breast carcinoma by Immunohistochemistry, Paraffin embedded tissue, in the absence and presence of the immunising peptide.

    References for Anti-Involucrin antibody (ab53112)

    This product has been referenced in:
    • Varone A  et al. Endogenous two-photon fluorescence imaging elucidates metabolic changes related to enhanced glycolysis and glutamine consumption in precancerous epithelial tissues. Cancer Res 74:3067-75 (2014). Read more (PubMed: 24686167) »
    • Ichida JK  et al. Notch inhibition allows oncogene-independent generation of iPS cells. Nat Chem Biol 10:632-9 (2014). Read more (PubMed: 24952596) »

    See all 3 Publications for this product

    Publishing research using ab53112? Please let us know so that we can cite the reference in this datasheet.

    Product Wall

    Submit an abreview for ab53112 Submit a question for ab53112

    8 Abreviews or Q&A

    10
    Votes: Highest First

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Involucrin antibody

     Excellent
    Abreviews
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (skin)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: citraconic anhydride
    Permeabilization
    No
    Specification
    skin
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10%
    Fixative
    Formaldehyde
    Read More
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    Username

    Abcam user community

    Verified customer

    Submitted Aug 01 2017

    Immunohistochemistry (Frozen sections) abreview for Anti-Involucrin antibody

     Excellent
    Abreviews
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Human Tissue sections (foreskin skin section)
    Permeabilization
    No
    Specification
    foreskin skin section
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 22°C
    Fixative
    none
    Read More
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    Username

    Mr. Julien Coutier

    Verified customer

    Submitted Jun 20 2017

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Involucrin antibody

     Good
    Abreviews
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (Skin)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: ph6
    Permeabilization
    No
    Specification
    Skin
    Blocking step
    BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 20°C
    Fixative
    Paraformaldehyde
    Read More
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    Username

    Abcam user community

    Verified customer

    Submitted Dec 15 2015

    Immunohistochemistry (Frozen sections) abreview for Anti-Involucrin antibody

     Below Average
    Abreviews
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Mouse Tissue sections (Skin)
    Permeabilization
    Yes - 0,3 % Triton X
    Specification
    Skin
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
    Fixative
    Perfusion fixed with paraformaldehyde and picric acid
    Read More
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    Username

    Abcam user community

    Verified customer

    Submitted Aug 12 2014

    We see no signal from capillary westerns of primary keratinocyte lysates or purified proteinat a 1/100 dilution. Please recommend an antibody that is capable of detecting involucrinfragment ab73744.
    Please recommend a membrane fractionation protoc...

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    Thank you for calling about the issue you are having with the involucrin antibody ab53112. The amino acid sequence of the recombinant fragment that you are using as a positive control, ab73744, overlaps the sequence of the immunogen at 11 of 14 amino a...

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    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Involucrin antibody

     Excellent
    Abreviews
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (Skin)
    Specification
    Skin
    Fixative
    Formaldehyde
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citric acid
    Permeabilization
    No
    Blocking step
    BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
    Read More
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    Username

    Mr. Carl Hobbs

    Verified customer

    Submitted Mar 19 2012

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Involucrin antibody

     Average
    Abreviews
    Abcam has not validated the combination of species/application used in this Abreview.
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (skin)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: H-3300 Vector lab
    Permeabilization
    No
    Specification
    skin
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
    Fixative
    Formaldehyde
    Read More
    abreview image
    Username

    Abcam user community

    Verified customer

    Submitted Feb 01 2012

    Dear Madam/Sir, regarding your inquiries these are my answers, 1) I tested the antibody on human cell lines oesophageal carcinoma , (KYSE450&OE21). 2)From Cork Cancer Research Center, CORK, IRELAND. 3) Please look to the attached file for my W.B (i...

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    Thank you for your reply with further information. The image suggests the higher band may be the correct band as it is only present in the first four samples as expected. You previously describe a higher band at 120 kDa and I assuming this is that b...

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