A synthetic peptide derived from human Involucrin.
Our Abpromise guarantee covers the use of ab53112 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
ICC/IF | Use at an assay dependent concentration. PubMed: 21067603 | |
WB | 1/300 - 1/1000. Detects a band of approximately 68 kDa (predicted molecular weight: 68 kDa). | |
IHC-P | Use at an assay dependent concentration. | |
ELISA | 1/20000. |
ab53112 staining human skin sections by IHC-P. The tissue was fixed with formaldehyde and a heat mediated antigen retrival step was performed with citric acid pH 6. Blocking of the sample was done with 1% BSA for 10 minutes at 21°C, followed by staining with ab53112 at 1/150 in TBS/BSA/azide for 16h at 21°C. A biotinylated goat anti-rabbit polyclonal antibody at 1/200 was used as the secondary antibody.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"