The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-P: use neat for 10 min at room temperature. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. In particular, boil tissue sections in 10mM citrate buffer, pH 6.0 for 10 min and then cool at room temperature for 20 min.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Part of the insoluble cornified cell envelope (CE) of stratified squamous epithelia.
Keratinocytes of epidermis and other stratified squamous epithelia.
Belongs to the involucrin family.
Substrate of transglutaminase. Some glutamines and lysines are cross-linked to other involucrin molecules, to other proteins such as keratin, desmoplakin, periplakin and envoplakin, and to lipids like omega-hydroxyceramide.
Cytoplasm. Constituent of the scaffolding of the cornified envelope.