Overview

  • Product name
    Anti-Involucrin antibody, prediluted
    See all Involucrin primary antibodies
  • Description
    Rabbit polyclonal to Involucrin, prediluted
  • Host species
    Rabbit
  • Specificity
    ab27496 recognises involucrin.
  • Tested applications
    Suitable for: IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment, corresponding to amino acids 420/578 of Human Involucrin

  • Positive control
    • Human skin

Properties

Applications

Our Abpromise guarantee covers the use of ab27496 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P
  • Application notes
    IHC-P: use neat for 10 min at room temperature. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. In particular, boil tissue sections in 10mM citrate buffer, pH 6.0 for 10 min and then cool at room temperature for 20 min.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Part of the insoluble cornified cell envelope (CE) of stratified squamous epithelia.
    • Tissue specificity
      Keratinocytes of epidermis and other stratified squamous epithelia.
    • Sequence similarities
      Belongs to the involucrin family.
    • Post-translational
      modifications
      Substrate of transglutaminase. Some glutamines and lysines are cross-linked to other involucrin molecules, to other proteins such as keratin, desmoplakin, periplakin and envoplakin, and to lipids like omega-hydroxyceramide.
    • Cellular localization
      Cytoplasm. Constituent of the scaffolding of the cornified envelope.
    • Information by UniProt
    • Database links
    • Alternative names
      • INVO_HUMAN antibody
      • Involucrin antibody
      • IVL antibody

    Images

    • Paraffin embedded formalin fixed human skin tissue section stained with anti Involucrin (ab27496).

    References

    This product has been referenced in:
    • Krishnamurthy S & Nör JE Orosphere assay: a method for propagation of head and neck cancer stem cells. Head Neck 35:1015-21 (2013). Read more (PubMed: 22791367) »

    See 1 Publication for this product

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab27496.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

    Sign up